全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

温度对茶小绿叶蝉成虫存活率及保护酶系的影响

, PP. 223-228

Keywords: 茶小绿叶蝉,温度,存活率,酶活力

Full-Text   Cite this paper   Add to My Lib

Abstract:

为探讨温度对茶小绿叶蝉适应能力的影响,室内设置-10、-5、0、5、10、15、20、25、30、35、38、41和44℃共13个温度梯度,测定了不同温度处理1h后成虫存活率及其保护酶系超氧化物歧化酶(SOD)、铜锌超氧化物歧化酶(CnZn-SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)和总蛋白含量的变化。结果表明,在5~35℃之间,成虫存活率均大于90%,其它处理成虫存活率明显降低。SOD、POD、CAT、T-AOC活性和总蛋白含量变化在5~30℃之间差异不显著,温度低于5℃或高于35℃时,保护酶活性亦有降低或增加的趋势,但均以41℃时含量最高,SOD、POD、CAT、T-AOC活性和总蛋白含量分别为2.64、0.68、1.58、0.67U/mg和178.33μg/mL,但CnZn-SOD活性在各处理中均无显著差异,其值在0.96~1.05U/mg之间。低温处理下的试虫反应更敏感,各指标含量均低于高温处理。表明茶小绿叶蝉成虫存活率、保护酶活性变化、总抗氧化能力和总蛋白含量与温度密切相关,在低温和高温下致死的原因与保护酶系统被破坏有关。

References

[1]  Abele D, Burlando B, Viarengo A, P?rtner HO. 1998. Exposure to elevated temperatures and hydrogen peroxide elicits oxidative stress and antioxidant response in the Antarctic intertidal limpet Nacella concinna. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 120(2): 425-435
[2]  An MI, Choi CY. 2010. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: effects on hemolymph and biochemical parameters. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 155(1): 34-42
[3]  Backus EA, Serrano MS, Ranger CM. 2005. Mechanisms of hopperburn: an overview of insect taxonomy, behavior and physiology. Annual Review of Entomology, 50: 125-151
[4]  Bale JS. 2002. Insects and low temperatures: from molecular biology to distributions and abundance. Philosophical Transactions of the Royal Society B: Biological Sciences, 357(1423): 849-862
[5]  Cao ML, Tao B, Liu S, Dong JG, He YZ. 2012. Influence of temperature on experimental population of Athetis lepigone (M?schler). Journal of Plant Protection, 39(6): 531-535 (in Chinese) [曹美琳, 陶晡, 刘顺, 董金皋, 何运转. 2012. 温度对二点委夜蛾实验种群的影响. 植物保护学报, 39(6): 531-
[6]  Datkhile KD, Mukhopadhyaya R, Dongre TK, Nath BB. 2009. Increased level of superoxide dismutase (SOD) activity in larvae of Chironomus ramosus (Diptera: Chironomidae) subjected to ionizing radiation. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(4): 500-506
[7]  Dubovskii IM, Grizanova EV, Chertkova EA, Slepneva IA, Komarov DA, Vorontsora YL, Glupov VV. 2010. Generation of reactive oxygen species and activity of antioxidants in hemolymph of the moth larvae Galleria mellonella (L.) (Lepidoptera: Piralidae) at development of the process of encapsulation. Journal of Evolutionary Biochemistry and Physiology, 46(1): 35-43
[8]  Feng AW, Zhang YP, Cen YJ, Liang GW. 2010. The control effect of ethanol extract of Mikania micrantha H.B.K. on the natural population of Empoasca vitis (G?the) and the influence to spiders. Journal of Environmental Entomology, 32(1): 54-59 (in Chinese) [冯安伟, 章玉苹, 岑伊静, 梁广文. 2010. 薇甘菊乙醇提取物对假眼小绿叶蝉自然种群的控制作用及其对蜘蛛的影响. 环境昆虫学报, 32(1): 54-
[9]  Ji R, Xin ZJ, Lou YG. 2011. Effects of temperature on growth, development, survival and reproduction of Corythucha ciliata (Say) (Hemiptera: Tingidae). Journal of Plant Protection, 38(2): 153-158 (in Chinese) [纪锐, 辛肇军, 娄永根. 2011. 温度对悬铃木方翅网蝽生长发育、存活和繁殖的影响. 植物保护学报, 38(2): 153-
[10]  Jin S, Sun XL, Chen ZM, Xiao B. 2012. Resistance of different tea cultivars to Empoasca vitis G?the. Scientia Agricultura Sinica, 45(2): 255-265 (in Chinese) [金珊, 孙晓玲, 陈宗懋, 肖斌. 2012. 不同茶树品种对假眼小绿叶蝉的抗性. 中国农业科学, 45(2): 255-
[11]  Li HL, Lin NQ. 2008. Studies on the biology of the egg parasitoids of tea leafhopper, Empoasca vitis (G?the). Journal of Tea Science, 28(6): 407-413 (in Chinese) [李慧玲, 林乃铨. 2008. 假眼小绿叶蝉卵缨小蜂的生物学特性研究. 茶叶科学, 28(6): 407-
[12]  Li ZZ, Shen HJ, Jiang QG, Ji BZ. 1994. A study on the activities of endogenous enzymes of protective system in some insects. Acta Entomologica Sinica, 37(4): 399-403 (in Chinese) [李周直, 沈惠娟, 蒋巧根, 稽保中. 1994. 几种昆虫体内保护酶系统活力的研究. 昆虫学报, 37(4): 399-
[13]  Liu CM. 2006. Effects of temperatures on survival rate and protection enzymes of Tenebrio molitor larva. Journal of Northwest Forestry University, 21(1): 107-109 (in Chinese) [刘缠民. 2006. 不同温度对黄粉虫幼虫存活率和保护酶系的影响. 西北林学院学报, 21(1): 107-
[14]  Liu CM, Ma JQ. 2006. Correlation analysis between survival rate and protection enzymes of Polyrhachis vicina under low temperatures. Journal of Northwest Forestry University, 21(6): 136-138 (in Chinese) [刘缠民, 马捷琼. 2006. 低温对大黑蚁存活率和保护酶影响的相关分析. 西北林学院学报, 21(6): 136-
[15]  Marnett LJ, Riggins JN, West JD. 2003. Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein. The Journal of Clinical Investigation, 111(5): 583-593
[16]  McDonald JR, Head J, Bale JS, Walters KF. 2000. Cold tolerance, overwintering and establishment potential of Trips palmi. Physiological Entomology, 25(2): 159-166
[17]  Mu D, Cui L, Ge J, Wang MX, Liu LF, Yu XP, Zhang QH, Han BY. 2012. Behavioral responses for evaluating the attractiveness of speci?c tea shoot volatiles to the tea green leafhopper, Empoasca vitis. Insect Science, 19(2): 229-238
[18]  Ni L, Dai RH. 2010. Scanning electron microscope observation on the antennal chemosensilla of three leafhopper species. Journal of Mountain Agriculture and Biology, 29(1): 88-90 (in Chinese) [倪林, 戴仁怀. 2010. 三种叶蝉触角化感器扫描电镜观察. 山地农业生物学报, 29(1): 88-
[19]  Park MS, Jo PG, Choi YK, An KW, Choi CY. 2009. Characterization and mRNA expression of Mn-SOD and physiological responses to stresses in the Pacific oyster Crassostrea gigas. Marine Biology Research, 5(5): 453-463
[20]  Peng P, Tang M, Hou YJ, Lin Q, Huang SJ, Deng M, Hu X, Zhang Y. 2010. Study on the effect and characters of yellow sticky trap sticking Aleurocanthus spiniferus and Empoasca vitis G?the in tea garden. Southwest China Journal of Agricultural Sciences, 23(1): 87-90 (in Chinese) [彭萍, 唐敏, 侯渝嘉, 林强, 黄尚俊, 邓敏, 胡翔, 张莹. 2010. 黄板诱杀茶园黑刺粉虱及假眼小绿叶蝉效果及特性研究. 西南农业学报, 23(1): 87-
[21]  更多...
[22]  Reineke A, Hauck M. 2012. Larval development of Empoasca vitis and Edwardsiana rosae (Homoptera: Cicadellidae) at different temperatures on grapevine leaves. Journal of Applied Entomology, 136(9): 656-664
[23]  Sibly RM, Calow P. 1989. A life-cycle theory of responses to stress. Biological Journal of the Linnean Society, 37(1/2): 101-116
[24]  Sinclair BJ, Vernon P, Klok CJ, Chown SL. 2003. Insects at low temperatures: an ecological perspective. Trends in Ecology and Evolution, 18(5): 257-262
[25]  Wang SG, Zhou Y, Feng LP. 2008. Food consumption, growth and activity of digestive enzymes of Catantops pinguis (St?l) fed on different diets under three temperatures. Journal of Plant Protection, 35(1): 1-6 (in Chinese) [王世贵, 周莹, 冯利苹. 2008. 温度和食物种类对红褐斑腿蝗取食、生长及肠道消化酶活性的影响. 植物保护学报, 35(1): 1-
[26]  Yang HM, Xie SX, Wang L, Jing SL, Zhu XP, Li XW, Zeng W, Yuan HY. 2011. Identi?cation of up-regulated genes in tea leaves under mild infestation of green leafhopper. Scientia Horticulturae, 130(2): 476-481
[27]  Yang TB, Mei SY. 1996. The effect of heat shock on the activities of antioxidative enzymes in silkworm (Bombyx mori). Progress in Biochemistry and Biophysics, 23(2): 153-156 (in Chinese) [杨唐斌, 梅尚筠. 1996. 热休克对家蚕幼虫抗氧化酶活性的影响. 生物化学与生物物理进展, 23(2): 153-
[28]  Yu Y, Xu HX, Zheng XS, Yang YJ, Lü ZX. 2013. Effects of drought stress on the ecological fitness of brown plant leafhopper, Nilaparvata lugens at high temperature. Journal of Plant Protection, 40(3): 193-199 (in Chinese) [于莹, 徐红星, 郑许松, 杨亚军, 吕仲贤. 2013. 在高温下干旱胁迫对褐飞虱生态适应性的影响. 植物保护学报, 40(3): 193-

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133