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黄瓜绿斑驳病毒河北分离物基因组克隆及序列分析

, PP. 182-187

Keywords: 黄瓜绿斑驳病毒,河北分离物,基因组,序列分析

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Abstract:

为揭示河北省黄瓜绿斑驳病毒(Cucumbergreenmottlemosaicvirus,CGMMV)分类地位及系统进化情况,通过分子克隆测定了来自河北省西瓜产区分离获得的分离物CGMMV-chb的近全基因组序列,分析其基因组结构特征;并依据近全基因组核苷酸序列及外壳蛋白氨基酸序列进行了系统进化关系分析。结果表明,CGMMV-chb近全基因组大小为6383nts,GenBank登录号为KJ658958,含4个开放阅读框,编码4种蛋白;与GenBank库中的其它12个CGMMV分离物的核苷酸相似性为92%~100%,氨基酸相似性为98%~100%。CGMMV-chb与韩国分离物CGMMV-KW基因序列相似性最高,为98%~100%,与以色列分离物CGMMVisolateEc基因序列相似性最低,为92%~99%;系统进化关系中CGMMV-chb与其它中国分离物、日韩分离物亲缘关系较近,处于同一亚组的不同分支中,与以色列分离物亲缘关系相对较远。

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