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人参病原菌拮抗细菌的分离筛选与鉴定

, PP. 79-86

Keywords: 人参,拮抗细菌,筛选,鉴定,定殖

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Abstract:

为明确土壤拮抗细菌对人参常见病原菌的生防价值,从吉林省抚松县人参根际土壤中分离、纯化获得113株细菌,采用平板对峙法测定菌株的拮抗能力,通过温室盆栽试验研究其在土壤及人参根部的定殖规律,并通过形态、培养特征及16SrDNA序列分析进行分类鉴定。对峙试验结果表明,2株细菌对供试人参病原菌具有良好的拮抗广谱性,且多次验证其抑菌效果稳定。定殖研究结果表明,活性菌株SZ-56与SZ-60的定殖能力均达到显著水平,在土壤中的最大定殖菌量分别为每克土7.51×106CFU和7.28×106CFU,30d后定殖菌量仍保持在105CFU;21d后在人参根部的定殖菌量仍保持在每克根105CFU水平。根据形态、培养特征、生理生化指标和16SrDNA序列分析,确定SZ-56为内生芽胞杆菌Bacillusendophyticus,SZ-60为解淀粉芽胞杆菌B.amyloliquefaciens。

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