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小麦成株期高温抗条锈基因Yrxy54-1的RGAP分子标记定位

, PP. 487-492

Keywords: 小偃54,高温成株抗条锈性,遗传分析,RGAP标记

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Abstract:

采用我国当前流行的7个小麦条锈菌(菌系),在高温条件下对其进行成株期抗条锈性鉴定,并用条锈菌CYR32对小麦品种小偃54与铭贤169的杂交后代及其双亲进行高温成株期抗条锈性遗传分析,以揭示小偃54成株期抗条锈性遗传机制。结果显示,小偃54成株期对多个条锈菌小种具有良好的抗病性,对CYR32的抗病性由2对隐性核基因独立控制,分别暂命名为Yrxy54-1和Yrxy54-2,并对Yrxy54-1进行分子标记定位。从528个RGAP引物组合中筛选到4个与抗病基因Yrxy54-1紧密连锁的多态性标记M1、M2、M3和M4,分布于Yrxy54-1的两侧,遗传距离分别为15.8、16.6、7.3和9.97?cM。遗传分析结合分子标记结果表明,Yrxy54-1是一个与已知抗条锈基因不同的新基因。

References

[1]  秦琳,李彬,崔凯荣,等. 小麦抗锈变异体的RAPD分子验证.西北植物学报,1997,17 (6):22-26
[2]  徐兵强,杜中军,黄俊生. RGA法克隆候选抗病基因的研究进展.分子植物育种,2004,2(3):421-428
[3]  Feuillet C, Reuzeau C, Kjellbom P, et al. Molecular characterization of a new type of receptor-like kinase (wlrk) gene family in wheat. Plant Molecular Biology, 1998, 37: 943-953
[4]  Leister D, Kurth J, Laurie D A, et al. Rapid reorganization of resistance gene homologues in cereal genomes. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95: 370-375
[5]  Donald T M, Pellerone F, Adam-Blondon A F, et al. Identification of resistance gene analogs linked to powdery mildew resistance locus in grapevine. Theoretical and Applied Genetics, 2002, 104: 610-618
[6]  Aarts M G M, te Lintel Hekkert B, Holub E B, et al. Identification of R-gene homologous DNA fragment genetically linked to disease resistance loci in Arabidopsis thaliana. Molecular Plant-Microbe Interactions, 1998, 11(4): 251-258
[7]  王关林,方宏筠. 植物基因工程(2版). 北京:科学出版社,2001
[8]  Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating segregation populations. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88: 9828-9832
[9]  Lin F, Chen X M. Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theoretical and Applied Genetics, 2007, 114: 1277-1287
[10]  Lin F, Chen X M. Molecular mapping of genes for race-specific overall resistance to stripe rust in wheat cultivar Express. Theoretical and Applied Genetics, 2008, 116: 797-806
[11]  Yan G P, Chen X M, Line R F, et al. Resistance gene-analog polymorphism markers co-segregating with the Yr5 [STBZ]gene for resistance to wheat stripe rust. Theoretical and Applied Genetics, 2003, 106: 636-643
[12]  Yan G P, Chen X M. Molecular mapping of a recessive gene for resistance to stripe rust in barley. Theoretical and Applied Genetics, 2006, 113: 529-537
[13]  杨作民,解超杰,孙其信. 后条中32时期我国小麦条锈抗源之现状.作物学报,2003,29(2):161-168
[14]  庄巧生. 中国小麦品种改良及系谱分析. 北京:中国农业出版社,2003
[15]  李振歧,商鸿生. 小麦锈病及其防治. 上海:上海科学技术出版社,1989
[16]  胡美君,王义芹,张亮,等. 不同基因型小麦及其优选杂交后代的光合作用特性. 作物学报,2007,33(11): 1879-1883
[17]  刘全有,童依平. "小偃54"优质小麦在豫南地区示范推广的抗逆性研究.中国生态农业学报,2002,10(3):62-64
[18]  吴会杰,魏学宁,周新力,等. 小偃54和小偃81高温抗条锈性及其与几丁质酶基因表达的关系.麦类作物学报,2007,27(6):1117-1122
[19]  吕伟东,徐鹏彬,蒲训. 偃麦草属种质资源在普通小麦育种中的应用现状简介.草业学报,2007,16(6):136-140[7] Zhang X Y, Dong Y S, Wang R R C. Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum×Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD. Genome, 1996, 39: 1062-1071
[20]  马渐新,周荣华,董玉琛,等. 来自长穗偃麦草的抗小麦条锈病基因的定位.科学通报,1999,44(1):65-69
[21]  更多...
[22]  Line R F, Qayoum A, Milus G. Durable resistance to stripe rust of wheat (Abstr.). Fourth International Congress Plant Pathology, Melbourne, Australia, 1983: 204
[23]  商鸿生. 小麦对条锈病的高温抗病性研究.中国农业科学,1998,31(4):46-50
[24]  陈国跃,董攀,魏育明,等. 普通小麦背景中长穗偃麦草Lophopyrum elongatum (Host) A. L?ve E染色体组的RGAP特异标记.作物学报,2007,33(11):1782-1787?

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