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红树内生细菌AmS2菌株对芒果炭疽病菌的抑制作用

, PP. 453-458

Keywords: 红树,内生细菌,鉴定,抗菌活性物质,芒果炭疽病菌

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Abstract:

为了解红树内生细菌AmS2菌株对芒果炭疽菌的抑菌机制及其分类学地位,采用生长速率法测定了菌株抗菌活性对芒果炭疽菌菌丝生长及孢子萌发的有效中浓度(EC50)。结果表明:经形态与培养特征观察、生理生化测定及16SrDNA序列分析,将AmS2菌株鉴定为解淀粉芽孢杆菌Bacillusamyloliquefaciens。抗菌活性物质分析发现,菌株可分泌产生易溶于水和甲醇等强极性溶剂、对热稳定、pH4~7条件下较稳定的非蛋白类抗菌活性物质。毒力测定表明,菌株抗菌物质粗提物对芒果炭疽病菌菌丝生长和分生孢子萌发的EC50分别为0.9772?mg/mL和1.9027?mg/mL,当提取物浓度?3.0?mg/mL时,可致使病菌分生孢子壁消解。

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