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北京地区地黄花叶病病原的分子鉴定

, PP. 447-452

Keywords: 蚕豆萎蔫病毒2,黄瓜花叶病毒,系统进化树

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Abstract:

为明确北京地区发生的地黄花叶病的病原,在进行生物学接种分离纯化的基础上利用RT-PCR方法对其进行了分子鉴定。通过接种指示植物和进行单斑分离,获得了病毒的纯分离物。经RT-PCR和序列测定及分析,有明显花叶症状的北京地黄样品受黄瓜花叶病毒Cucumbermosaicvirus(CMV)和蚕豆萎蔫病毒2Broadbeanwiltvirus2(BBWV2)复合侵染。为进一步明确BBWV2地黄分离物(BBWV2-Rg)的分类地位,克隆了BBWV2-RgRNA2多聚蛋白基因,并进行了序列测定和分析,结果表明该多聚蛋白基因由3?195个核苷酸组成,编码1?064个氨基酸。经序列比对分析,BBWV2-Rg编码的外壳蛋白大亚基LCP基因和小亚基SCP基因核苷酸序列与已发表的BBWV2其它株系相应基因核苷酸序列的同源性分别为78.69%~89.30%和76.99%~90.52%,氨基酸序列同源性分别为91.29%~97.51%和87.82%~96.45%。

References

[1]  Srinivasa M V. Host plants of the spiraling whitefly Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae). Pest Management in Horticultural Ecosystems, 2000, 6: 79-105
[2]  Mware B, Olubayo F, Narla R, et al. First record of spiraling whitefly in Coastal Kenya: emergence, host range, distribution and association with cassava brown streak virus disease. International Journal of Agriculture and Biology, 2010, 12: 411-415?
[3]  韩冬银, 刘奎, 陈伟, 等. 螺旋粉虱在海南的分布与寄主植物种类调查. 昆虫知识, 2008, 45(5): 765-770
[4]  Russell L M. A new species of Aleurodicus Douglas and two close relatives. Florida Entomologist, 1965, 48: 47-55?
[5]  Lambkin T A. A host list for Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae) in Australia. Australian Journal of Entomology, 1999, 38: 373-376?
[6]  Neuenschwander P. Spiraling whitefly, Aleurodicus dispersus, a recent invader and new cassava pest. African Crop Science Journal, 1994, 2(4): 419-421?
[7]  D′Almeida Y A, Lys J A, Neuenschwander P, et al. Impact of two accidentally introduced Encarsia species (Hymenoptera: Aphelinidae) and other biotic and abiotic factors on the spiraling whitefly Aleurodicus dispersus (Russell) (Homoptera: Aleyrodidae), in Benin, West Africa. Biocontrol Science and Technology, 1998, 8: 163-173?
[8]  Ramani S, Poorani J, Bhumannavar B S. Spiraling whitefly, Aleurodicus dispersus, in India. Biocontrol News and Information, 2002, 23: 55-62
[9]  刘循, 万方浩, 张桂芬. 可用于黑刺粉虱快速鉴定的SCAR分子标记技术. 昆虫学报, 2009, 52(8): 895-900?
[10]  更多...
[11]  孟祥钦, 闵亮, 万方浩, 等. 西花蓟马的SCAR分子检测技术. 昆虫学报, 2010, 53(3): 323-330?
[12]  Walsh K, Boonham N, Barker I, et al. Development of a sequence?specific real?time PCR to the melon thrips Thrips palmi (Thysan., Thripidae). Journal of Applied Entomology, 2005, 129(5): 272-279?
[13]  Kethidi D R, Roden D B, Ladd T R, et al. Development of SCAR markers for the DNA?based detection of the Asian long?horned beetle, Anoplophora glabripennis (Motschulsky). Archives of Insect Biochemistry and Physiology, 2003, 52: 193-204?
[14]  罗晨, 姚远, 王戎疆, 等. 利用mtDNA CO I基因序列鉴定我国烟粉虱的生物型. 昆虫学报, 2002, 45(6): 759-763?
[15]  周志湘, 万方浩, 张桂芬, 等. 烟粉虱基因组DNA快速提取方法. 植物保护, 2007, 33(5): 131-133?
[16]  Alomar O, Castane C, Cabarra R, et al. Mirid bugs ? Another strategy for IPM on Mediterranean vegetable crops? Bulletin SROP, 1990, 13: 6-9?
[17]  Gerling D. Approaches to the biological control of whiteflies. Florida Entomologist, 1992, 75: 446-454?
[18]  Zhang G F, Lü Z C, Wan F H, et al. Real?time PCR quantification of Bemisia tabaci? (Homoptera: Aleyrodidae) B?biotype remains in predator guts. Molecular Ecology Notes, 2007, 7: 947-954
[19]  Banjo A D. A review of on Aleurodicus dispersus Russell (spiraling whitefly) (Hemiptera: Aleyrodidae) in Nigeria. Journal of Entomology and Nematology, 2010, 2(1): 1-6?
[20]  徐岩. 警惕螺旋粉虱传入中国. 植物检疫, 1999, 13(4): 232-236?
[21]  虞国跃, 张国良, 彭正强, 等. 螺旋粉虱入侵我国海南. 昆虫知识, 2007, 44(3): 428-431
[22]  韩冬银, 刘奎, 张方平, 等. 螺旋粉虱的生物学特性. 昆虫学报, 2009, 52(3): 281-289?
[23]  De Barro P J, de Driver F. Use of RAPD PCR to distinguish the B biotype from other biotypes of Bemisia tabaci? (Gennadius) (Hemiptera: Aleyrodidae). Australian Journal of Entomology, 1997, 36: 149-152?
[24]  De Barro P J, de Liebregts W, Carver M. Distribution and identity of biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in member countries of the Secretariat of the Pacific Community. Australian Journal of Entomology, 1998, 37: 214-218?
[25]  Qiu B L, Ren S X, Wen S Y, et al. Biotype identification of the populations of Bemisia tabaci (Homoptera: Aleyrodidae) in China using RAPD?PCR. Acta Entomologica Sinica, 2003, 46: 605-608?
[26]  Callejas C, Velasco A, Gobbi A, et al. Fast discrimination (RAPD?PCR) of the species forming the pest complex Aleurodicus dispersus?Lecanoideus floccissimus (Hom: Aleyrodidae). Journal of Applied Entomology, 2005, 129(7): 382-385?
[27]  Li Z X, Hu D X. Rapid identification of B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) based on analysis of internally transcribed spacer 1 sequence. Insect Science, 2005, 12: 421-427?
[28]  臧连生, 江彤, 徐婧, 等. 烟粉虱B型及二个非B型种群的SCAR分子标记. 农业生物技术学报, 2006, 14(2): 208-212?
[29]  Zhang G F, Lü Z C, Wan F H. Detection of Bemisia tabaci remains in predator guts using a sequence?characterized amplified region marker. Entomologia Experimentalis et Applicata, 2007, 123: 81-90?

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