全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

螺旋粉虱SCAR标记的建立与应用

, PP. 385-390

Keywords: 螺旋粉虱,SCAR标记,分子检测,特异性扩增,口岸检疫

Full-Text   Cite this paper   Add to My Lib

Abstract:

螺旋粉虱AleurodicusdispersusRussell是一种严重威胁果树、蔬菜和园林植物生产的入侵性害虫。由于该粉虱的卵和初孵若虫体型微小,与其它粉虱类害虫形态相似,且易于携带传播,难以快速准确鉴别,本研究采用特征序列扩增区域(SCAR)标记法,通过对目的片段的克隆与测序,设计螺旋粉虱特征片段扩增引物,并对该引物的种特异性和灵敏性进行检验。结果显示,利用SCAR标记法获得了螺旋粉虱特异性片段(632?bp,GenBank登录号为HM240855),根据此片段的碱基序列设计1对螺旋粉虱特异性引物,其扩增片段大小为307?bp;该对引物只对螺旋粉虱具有扩增能力,对近缘种、属粉虱不具有扩增效果。该引物不仅对成虫具有良好的扩增效能,对单粒卵、1~3龄若虫和拟蛹等亦具有同样的扩增能力,其最低检出阈值为1/6?400头成虫。从而表明,该SCAR标记技术完全可用于螺旋粉虱的鉴定识别及其检测和监测。

References

[1]  De Barro P J, de Driver F. Use of RAPD PCR to distinguish the B biotype from other biotypes of Bemisia tabaci? (Gennadius) (Hemiptera: Aleyrodidae). Australian Journal of Entomology, 1997, 36: 149-152?
[2]  De Barro P J, de Liebregts W, Carver M. Distribution and identity of biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in member countries of the Secretariat of the Pacific Community. Australian Journal of Entomology, 1998, 37: 214-218?
[3]  Srinivasa M V. Host plants of the spiraling whitefly Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae). Pest Management in Horticultural Ecosystems, 2000, 6: 79-105
[4]  钱景秦, 周?镒, 张淑贞. 螺旋粉虱(?Aleurodicus dispersus?)之发生与生物防治. 台湾昆虫, 2002, 特刊3(农作物害虫与害螨生物防治研讨会): 93-109?
[5]  Mware B, Olubayo F, Narla R, et al. First record of spiraling whitefly in Coastal Kenya: emergence, host range, distribution and association with cassava brown streak virus disease. International Journal of Agriculture and Biology, 2010, 12: 411-415?
[6]  韩冬银, 刘奎, 陈伟, 等. 螺旋粉虱在海南的分布与寄主植物种类调查. 昆虫知识, 2008, 45(5): 765-770
[7]  Russell L M. A new species of Aleurodicus Douglas and two close relatives. Florida Entomologist, 1965, 48: 47-55?
[8]  Lambkin T A. A host list for Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae) in Australia. Australian Journal of Entomology, 1999, 38: 373-376?
[9]  Neuenschwander P. Spiraling whitefly, Aleurodicus dispersus, a recent invader and new cassava pest. African Crop Science Journal, 1994, 2(4): 419-421?
[10]  D′Almeida Y A, Lys J A, Neuenschwander P, et al. Impact of two accidentally introduced Encarsia species (Hymenoptera: Aphelinidae) and other biotic and abiotic factors on the spiraling whitefly Aleurodicus dispersus (Russell) (Homoptera: Aleyrodidae), in Benin, West Africa. Biocontrol Science and Technology, 1998, 8: 163-173?
[11]  Ramani S, Poorani J, Bhumannavar B S. Spiraling whitefly, Aleurodicus dispersus, in India. Biocontrol News and Information, 2002, 23: 55-62
[12]  Banjo A D. A review of on Aleurodicus dispersus Russell (spiraling whitefly) (Hemiptera: Aleyrodidae) in Nigeria. Journal of Entomology and Nematology, 2010, 2(1): 1-6?
[13]  徐岩. 警惕螺旋粉虱传入中国. 植物检疫, 1999, 13(4): 232-236?
[14]  虞国跃, 张国良, 彭正强, 等. 螺旋粉虱入侵我国海南. 昆虫知识, 2007, 44(3): 428-431
[15]  韩冬银, 刘奎, 张方平, 等. 螺旋粉虱的生物学特性. 昆虫学报, 2009, 52(3): 281-289?
[16]  Qiu B L, Ren S X, Wen S Y, et al. Biotype identification of the populations of Bemisia tabaci (Homoptera: Aleyrodidae) in China using RAPD?PCR. Acta Entomologica Sinica, 2003, 46: 605-608?
[17]  Callejas C, Velasco A, Gobbi A, et al. Fast discrimination (RAPD?PCR) of the species forming the pest complex Aleurodicus dispersus?Lecanoideus floccissimus (Hom: Aleyrodidae). Journal of Applied Entomology, 2005, 129(7): 382-385?
[18]  Li Z X, Hu D X. Rapid identification of B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) based on analysis of internally transcribed spacer 1 sequence. Insect Science, 2005, 12: 421-427?
[19]  臧连生, 江彤, 徐婧, 等. 烟粉虱B型及二个非B型种群的SCAR分子标记. 农业生物技术学报, 2006, 14(2): 208-212?
[20]  Zhang G F, Lü Z C, Wan F H. Detection of Bemisia tabaci remains in predator guts using a sequence?characterized amplified region marker. Entomologia Experimentalis et Applicata, 2007, 123: 81-90?
[21]  更多...
[22]  刘循, 万方浩, 张桂芬. 可用于黑刺粉虱快速鉴定的SCAR分子标记技术. 昆虫学报, 2009, 52(8): 895-900?
[23]  孟祥钦, 闵亮, 万方浩, 等. 西花蓟马的SCAR分子检测技术. 昆虫学报, 2010, 53(3): 323-330?
[24]  Walsh K, Boonham N, Barker I, et al. Development of a sequence?specific real?time PCR to the melon thrips Thrips palmi (Thysan., Thripidae). Journal of Applied Entomology, 2005, 129(5): 272-279?
[25]  Kethidi D R, Roden D B, Ladd T R, et al. Development of SCAR markers for the DNA?based detection of the Asian long?horned beetle, Anoplophora glabripennis (Motschulsky). Archives of Insect Biochemistry and Physiology, 2003, 52: 193-204?
[26]  罗晨, 姚远, 王戎疆, 等. 利用mtDNA CO I基因序列鉴定我国烟粉虱的生物型. 昆虫学报, 2002, 45(6): 759-763?
[27]  周志湘, 万方浩, 张桂芬, 等. 烟粉虱基因组DNA快速提取方法. 植物保护, 2007, 33(5): 131-133?
[28]  Alomar O, Castane C, Cabarra R, et al. Mirid bugs ? Another strategy for IPM on Mediterranean vegetable crops? Bulletin SROP, 1990, 13: 6-9?
[29]  Gerling D. Approaches to the biological control of whiteflies. Florida Entomologist, 1992, 75: 446-454?
[30]  Zhang G F, Lü Z C, Wan F H, et al. Real?time PCR quantification of Bemisia tabaci? (Homoptera: Aleyrodidae) B?biotype remains in predator guts. Molecular Ecology Notes, 2007, 7: 947-954

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133