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二斑叶螨对阿维菌素的抗药性及抗性基因的PASA检测技术

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Keywords: 二斑叶螨,阿维菌素,抗药性,谷氨酸门控氯离子通道,G323D点突变

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Abstract:

为了明确二斑叶螨TetranychusurticaeKoch对阿维菌素的抗性水平,采用玻片浸渍法测定了北京4个地区二斑叶螨雌成螨对阿维菌素的抗药性,建立了特异性等位基因PCR(PASA)方法,并检测了二斑叶螨谷氨酸门控氯离子通道(GluCl)基因片段上G323D的突变频率。结果显示,北京昌平、海淀、密云和怀柔4个田间二斑叶螨种群对阿维菌素均达极高抗性水平,其中昌平种群抗性最高,LC50值为448.93mg/L,抗性倍数为4988.11倍。室内敏感种群未见抗性个体,昌平和密云种群G323D等位基因突变频率为100.00%,怀柔和海淀种群分别为86.25%和90.00%。北京4个地区二斑叶螨种群对阿维菌素达极高抗性水平,抗性基因的突变频率也很高,表明阿维菌素不适宜用来防治这些地区的二斑叶螨种群。

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