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基于叶绿体matK基因序列探讨10种石杉科石杉属植物的系统关系及分子鉴定

Keywords: 石杉属,matK基因,系统发育,分子鉴定

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Abstract:

目的:利用叶绿体matK基因序列探讨10种石杉属植物的系统发育及分子鉴定。方法:分别从9种石杉属植物中提取总DNA,经扩增纯化后,用PCR产物直接测序。结果:对石杉属HuperziaBernh.9个种的叶绿体matK基因进行了测序,序列长度为1589bp,并对10个种(亮叶石杉H.lucidula的matK基因序列从GenBank中下载)的matK序列进行比对,其中有35个系统发育信息位点及35个变异位点,以垂穗石松Lycopodiellacernua为外类群,利用MEGA3.1软件计算种与种之间的遗传距离在1.59%~0.25%,并构建系统树,获得最简约树和邻接树。结论:小杉兰组和蛇足石杉组植物分别聚成一枝,构成姊妹群,其靴带支持率分别为98%和99%。长柄石杉与蛇足石杉分别聚在2个不同进化枝,二者间有19个碱基差异,遗传距离为1.21%。建议应将长柄石杉单独列为一个种。

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