全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

不同生长阶段线虫衰老相关基因的表达变化及川芎提取物的调控作用

Keywords: 川芎提取物,秀丽隐杆线虫,寿命,衰老,基因表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:观察不同生长阶段秀丽隐杆线虫衰老相关基因的表达变化及川芎提取物(CXE)的调控作用。方法:按生长阶段不同分设2,6d2个时间点,采用实时荧光定量RT-PCR(qRT-PCR)方法检测上述时间点对照组和用药组的衰老相关基因表达情况,以2-ΔΔCT法比较分析其基因表达变化的差异及CXE的调控作用。结果:与2d对照组比较,6d对照组线虫的age-1,daf-2,let-363基因表达上调(P<0.05),而ins-18,let-60,sir-2.1,sod-3基因表达下调(P<0.05);与用药2d组比较,用药6d组age-1,daf-2,let-363基因表达显著下调(P<0.01),ins-18,let-60,sir-2.1,sod-3基因表达显著上调(P<0.01)。结论:age-1,daf-2,let-363基因进入衰老期后表达量增加,显示促进衰老作用;而ins-18,let-60,sir-2.1,sod-3进入衰老期后表达量下降,显示延缓衰老的作用。CXE主要通过抑制胰岛素信号通路和限食等通路的衰老基因表达,促进长寿基因表达发挥延寿作用。

References

[1]  王学波,赵琪.衰老过程中线粒体DNA数量变化观察[J].中国误诊学杂志,2007,7(7):1490.
[2]  汪晓燕,王香明,王丹巧. 川芎提取物对线虫寿命的影响及分子机制研究[J].中国中药杂志,2010, 35(8):1042.
[3]  蔡外娇,张新民,黄建华,等.淫羊藿总黄酮延缓秀丽隐杆线虫衰老的实验研究[J].中国中西医结合杂志,2008,28(6):522.
[4]  Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-ΔΔCT ) method[J]. Methods, 2001,25:402.
[5]  马永兴,俞卓伟.现代衰老学[M].北京:科学技术文献出版社,2008:471.
[6]  Kayo Yasuda, Takamasa Ishii, Hitoshi Suda, et al. Age-related changes of mitochondrial structure and function in Caenorhabditis elegans[J]. Mech Ageing Dev, 2006,127:763.
[7]  Beckman K B, Ames B N. The free radical theory of aging matures[J].Physiology,1998, 78:547.
[8]  Harriet Gershon, David Gershon. Caenorhabditis elegans-a paradigm for aging research: Advantages and limitations[J]. Mech Ageing Dev, 2002,123: 261.
[9]  Vanfleteren J R, Braeckman B P. Mechanisms of life span determination in Caenorhabditis elegans[J]. Neurobiol Aging, 1999,20: 487.
[10]  Tsui D, van der Kooy D. Serotonin mediates a learned increase in attraction to high concentrations of benzaldehyde in aged Caenorhabditis elegans[J]. Learn Mem, 2008, 15(11):844.
[11]  Browner WS.The genetics of human longevity[J]. Am J Med, 2004, 117(11): 851.
[12]  Jason Z Morris,Heidi A Tissenbaum, Garry Ruvkun. A phosphotidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans[J]. Nature, 1996, 382: 536.
[13]  Pierce S B, Costa M, Wisotzkey R, et al. Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family[J]. Gene Dev, 2001,15(6):672.
[14]  Tissenbaum H A, Guarente L. Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans[J]. Nature,2001,410: 227.
[15]  Yoko Honda, Shuji Honda. The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans[J]. FASEB J, 1999,13:1385.
[16]  Manoj Nanji, Neil A Hopper, David Gems. LET-60 RAS modulates effects of insulin/IGF-1 signaling on development and aging in Caenorhabditis elegans[J]. Aging Cell,2005,4: 235.
[17]  Masoro E J.Overview of caloric restriction and ageing[J]. Mech Aging Dev, 2005,126:913.
[18]  Yamei Wang, Heidi A Tissenbaum. Overlapping and distinct functions for a Caenorhabditis elegans SIR2 and DAF-16/FOXO[J]. Mech Aging Dev,2006,127: 48.
[19]  Vellai T, Takacs-Vellai K, Zhang Y, et al. Genetics: Influence of TOR kinase on lifespan in C. elegans[J]. Nature, 2003, 426: 620.
[20]  Kailiang Jia, Di Chen, Donald L Riddle. The TOR pathway interacts with the insulin signaling pathway to regulate C. elegans larval development, metabolism and life span[J]. Development,2004,131: 3897.
[21]  刘小雨,王琦,夏世金,等. 增龄进程淋巴细胞核转录因子κB相关基因表达及淫羊藿总黄酮的调控作用[J].中医杂志,2009,50(1):66.
[22]  黄华永,沈海星,郑锦鸿.阿魏酸及其类似物的合成及其清除自由基活性研究[J].中国新药杂志, 2006 (6): 236.
[23]  王丹巧,王巍,景富春,等.川芎嗪对帕金森病大鼠脑内灌流左旋多巴引起的脑氧化损伤的影响[J].中国中西医结合杂志,2007,27(7):629.
[24]  徐升强,贾岷.川芎嗪对急性胰腺炎大鼠细胞凋亡的影响[J].微循环学杂志,2009,19(2):25.
[25]  杨静,雷燕,崔巍,等.人参三七川芎提取物延缓内皮细胞复制性衰老的机制研究[J].中国中药杂志, 2009, 34(12): 1544.
[26]  于文涛,张一昕,吴中秋,等.补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响[J].中国老年学杂志,2009,29:1478.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133