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黄芪总苷防治地塞米松诱导小鼠记忆障碍和对APP及β-分泌酶mRNA表达的研究

Keywords: 黄芪总苷,糖皮质激素,记忆障碍,淀粉样前体蛋白,α-分泌酶,β-分泌酶

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Abstract:

目的:探讨黄芪总苷(AST)对地塞米松(DEX)诱导小鼠记忆障碍的保护作用及对脑内淀粉样前体蛋白(APP)及其mRNA,α-分泌酶和β-分泌酶mRNA表达的影响。方法:将小鼠随机分成6组:正常对照组、模型组、AST(10,20,40mg·kg-1)组和阳性药(人参皂苷Rg1,6.5mg·kg-1)组。用DEX(5mg·kg-1,ig,21d)建立小鼠学习记忆损伤模型,各用药组于造模同时ig给予相应药物,模型组和对照组ig等容积蒸馏水。用Morris水迷宫方法测定小鼠学习记忆功能;用RT-PCR方法检测脑组织中APP、α-分泌酶和β-分泌酶mRNA表达;用免疫组织化学方法观察大脑皮质,海马CA1,CA3区APP表达。结果:与模型组比较,AST(20,40mg·kg-1)能明显改善小鼠记忆功能(P<0.05,P<0.01);降低脑组织内APP,β-分泌酶mRNA的表达(P<0.05),升高脑组织内α-分泌酶mRNA的表达(P<0.05);减少APP在大脑皮质及海马CA1区的表达(P<0.05)。结论:AST能改善DEX诱导的小鼠记忆障碍,其机制可能与抑制脑内APP及其mRNA、β-分泌酶mRNA表达,促进α-分泌酶mRNA表达有关。

References

[1]  Hoschlc, Hajek T. Hippocampal damage mediated by corticosteroids-a neuropsychiatric research challenge[J]. Eur Arch Psychiatry Clin Neurosci, 2001, 251 (2) : II/81.
[2]  李维祖, 李卫平, 尹艳艳, 等. 黄芪总苷及黄芪甲苷对糖皮质激素诱导老前期小鼠记忆障碍的保护作用及其机制[J].安徽医药,2008,12 (7 ): 592.
[3]  李维祖, 李卫平, 尹艳艳. 黄芪总苷及黄芪甲苷对糖皮质激素诱导衰老大鼠氧自由基代谢的影响[J].中国中药杂志,2007,32 (23 ): 2539.
[4]  Mattson M. Pathways towards and away from Alzheimer’s disease[J]. Nature, 2004, 430 (7000): 631.
[5]  Green K N, Billings L M, Roozendaal B, et al. Glucocorticoids increase amyloid-β and tau pathology in a mouse model of Alzheimer’s disease[J]. J Neurosci, 2006, 26 (35): 9047.
[6]  Catania C, Sotiropoulos I, Silva R, et al. The amyloidogenic potential and behavioral correlates of stress[J].Mol Psychiatry,2009,14 (1): 95.
[7]  任玮, 左丽, 钟志强. 灵芝孢子粉对糖皮质激素抑制模型小鼠的免疫调节作用[J].中国免疫学杂志,2007, 23 (11): 979.
[8]  史传道, 汶勇军, 文鹏, 等. 抗疏健骨颗粒对糖皮质激素骨质疏松大鼠骨密度和血清IL - 6的影响[J]. 陕西中医学院学报, 2007, 30 (5): 63.
[9]  McIntosh L J, Hong K E, Sapolsky R M. Glucocorticoids may alter antioxidant enzyme capacity in the brain: Baseline studies[J]. Brain Res, 1998, 791 (1/2) : 209.
[10]  Yao Y Y, Liu D M, Xu D F, et al . Memory and learning impairment induced by dexamethasone in senescent but not young mice[J]. Eur J Pharmacol, 2007, 574(1):20.
[11]  Swaab D F, Bao A M, Lucassen P J. The stress system in the human brain in depression and neurodegeneration[J]. Ageing Res Rev, 2005,4 (2):141.
[12]  Raber J. Detrimental effects of chronic hypothalamic-pituitary adrenal axis activation.From obesity to memory deficits[J]. Mol Neurobiol, 1998, 18 (1): 1.
[13]  Arborelius L, Owens M J, Plotsky P M, et al. The role of corticotropin-releasing factor in depression and anxiety disorders[J]. J Endocrinol, 1999, 160 (1): 1.
[14]  Davis K L, Davis B M, Greenwald B S, et al. Cortisol and Alzheimer\'s disease,I:Basal studies[J]. Am J Psychiatry,1986,143 (3): 300.
[15]  Masugi F, Ogihara T, Sakaguchi K, et al. High plasma levels of cortisol in patients with senile dementia of the Alzheimer\'s type[J]. Methods Find Exp Clin Pharmacol,1989,11 (11): 707.
[16]  Swanwick G R, Kirby M, Bruce I, et al. Hypothalamic-pituitary-adrenal axis dysfunction in Alzheimer’s disease: Lack of association between longitudinal and cross-sectional findings[J]. Am J Psychiatry,1998,155 (2): 286.
[17]  Hardy J. Amyloid, the presenilins and Alzheimer\'s disease[J]. Trends Neurosci, 1997, 20 (4): 154.
[18]  Stein T D, Anders N J, De Carli C, et al. Neutralization of transthyretin revers the neuroprotective effects of secreted amyloid precursor protein (APP) in APPsw mice resulting in tau hosphorylation and loss of hippocampalneurons:Support for the amyloid hypothesis[J]. J Neosci,2004,24 (35): 7707.
[19]  Lei H, Wang B, Li W P, et al. Anti-aging effect of astragalosides and its mechanism of action[J]. Acta Pharmacol Sin, 2003, 24 (3): 230.
[20]  路景涛, 陈敏珠. 复方黄芪提取物对细菌脂多糖诱导大鼠腹腔巨噬细胞分泌TNFα,NO及IL-1的影响[J].安徽医药, 2006, 10 (5): 330.

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