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福建金线莲与菌根真菌互作过程中的蛋白质组研究

Keywords: 金线莲,菌根真菌,蛋白质组,双向电泳

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Abstract:

目的:通过蛋白质组差异研究菌根真菌促进植物生长的机制。方法:采用蛋白质双向电泳结合MALDI-TOF/TOF质谱方法研究了接种瘤菌根菌属Epulorhizasp.的福建金线莲的蛋白组。结果:MALDI-TOF/TOF质谱分析了27个差异蛋白点,在数据库中以绿色植物为检索范围,确定了22个差异蛋白,功能大多涉及植物的信号传导、代谢调节等,光合作用及物质代谢中的功能蛋白及酶类等也有涉及。结论:菌根真菌通过影响植物的调控系统作用于植物,积极的调控使得植物物质代谢、光合作用等增强,进而表现出植株健壮、抗性增强;研究还表明植物与菌根真菌互作时可能发生某些基因的沉默。

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