蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的含量

目的:建立蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的HPLC含量测定法,并对蟾酥、蟾皮、蟾衣中2类成分进行分析。方法:吲哚生物碱类,NucleosilC18柱,乙腈-0.5%磷酸二氢钾溶液(6:94,磷酸调pH3.2)为流动相,流速0.8mL·min-1,检测波长275nm,柱温30℃;蟾毒配基类,AlltimaC18柱,乙腈(A)-0.3%乙酸溶液(B)为流动相,梯度洗脱(0~15min,28%~54%A;15~35min,54%A),流速0.6mL·min-1,检测波长296nm,柱温30℃。结果:五羟色胺、N-甲基五羟色胺、N,N-二甲基五羟色胺、N,N,N-三甲基五羟色胺、蟾蜍噻咛依次在0.0796~0.796,0.0972~1.945,0.0744~0.744,0.103~2.05,0.0672~0.672μg线性关系良好;五羟色胺和N-甲基五羟色胺的平均加样回收率依次为98.6%和91.3%;日蟾毒它灵、蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基依次在0.00483~0.614,0.0079~1.006,0.00795~1.016,0.0097~1.24,0.0096~1.22μg线性关系良好;平均加样回收率依次为101.6%,102.5%,101.0%,99.1%,98.9%。结论:蟾酥中吲哚生物碱和蟾毒配基类成分含量均较高,蟾皮中含量次之,蟾衣中含量最低甚至检测不到。