全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

外源Ca2+,ALA,SA和Spd对盐胁迫下紫苏种子萌发及幼苗生理特性的影响

Keywords: 紫苏,钙离子,ALA,SA,Spd,盐胁迫,种子萌发,生理特性

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:通过对紫苏种子萌发及幼苗生理特性的研究,寻找提高紫苏种子及幼苗在盐胁迫条件下抗性能力的途径。方法:测定NaCl胁迫下紫苏种子在不同浓度的外源钙离子(Ca2+)、5-氨基乙酰丙酸(ALA)、水杨酸(SA)和亚精胺(Spd)处理后,发芽势(Gv)、发芽率(Gr)、发芽指数(Gi)和活力指数(Vi)的变化。并对紫苏幼苗总生物量以及叶片丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性进行测定。结果:100mmol·L-1的NaCl胁迫下的紫苏种子萌发受到显著抑制,但是经过Ca2+,ALA,SA,Spd处理后,萌发指标均有升高。外施Ca2+(10mmol·L-1),ALA(100mg·L-1),SA(50mg·L-1)或Spd(0.25mmol·L-1)都能够有效的缓解NaCl对紫苏种子及幼苗造成的胁迫伤害,其中经过100mg·L-1的ALA处理后,效果最为显著,各项指标均达到最大值,发芽势为65.3%,发芽率为89.7%,发芽指数为15.2,活力指数为为0.1238。各处理均提高了幼苗的总生物量,降低了叶片的MDA含量,显著的提高了SOD,POD,CAT的活性,经过100mg·L-1的ALA处理后3种酶的活性分别达到最大值(0.72,6.82,5.64U·mg-1)。结论:适宜浓度的Ca2+,ALA,SA,Spd都能够有效的减缓NaCl胁迫对紫苏种子及幼苗产生的伤害,提高种子及幼苗的抗盐能力。

References

[1]  Y Hotta, T Tanaka, H Takaoka, et al. Promotive effects of 5-aminolevulinic acid on the yield of several crops[J]. Plant Growth Regul, 1997,22:109.
[2]  Watanabe K, Tanaka T, Hotty A, et al. Improving salt tolerance of cotton seedlings with 5-aminolevulinic acid[J]. Plant Growth Regul, 2000,32:99.
[3]  Wang L J, Jiang W B, Liu H, et al. Promotion of 5-aminolevulinic acid (ALA) on germination of pakchoi (Brassica chinensis) seeds under salt stress[J]. J Integr Plant Biol, 2005,47(9):1084.
[4]  沈文飚,徐朗莱,叶茂炳.水杨酸诱导植物抗病性的新进展[J].生物化学与生物物理进展,1999,26(3): 237.
[5]  姜中珠,陈祥伟.水杨酸对三种灌木幼苗抗旱性的影响[J].水土保持学报,2004,18(2):166,185.
[6]  林忠平,胡鸢雷.植物抗逆性与水杨酸介导的信号传导途径的关系[J].植物学报,1997,39(2):185.
[7]  梁洁,严重玲,李裕红,等.Ca(NO3)2对NaCl胁迫下麻黄扦插苗生理特征的调控[J].生态学报,2004,24(5):1073.
[8]  Hetherington A M, Brownlee C. The generation of Ca2+ signals in plants [J]. Annu Rev Plant Biol, 2004,55: 401.
[9]  Zhao X, Wang Y L, Wang Y J, et al. Effects of exogenous Ca2+on stomatal movement and plasma membrane K+ channels of Vicia faba guard cell under salt stress[J]. Acta agronomica sinica, 2008,34: 1970.
[10]  Zhu J K. Regulation of ion homeostasis under salt stress [J]. Curr Opin Plant Biol, 2003,6: 441.
[11]  Epstein E. How calcium enhances plant salt tolerance [J]. Science, 1998,280: 1906.
[12]  Rus A, Lee B H, Mayor A M, et al. AtHKT1 facilitates Na+ homeostasis and K+ nutrition in planta [J].J Plant Phsiol, 2004 ,136: 2500.
[13]  李璟,胡晓辉,郭世荣,等.外源亚精胺对根际低氧胁迫下黄瓜幼苗根系多胺含量和抗氧化酶活性的影响[J].植物生态学报,2006,30(1):118.
[14]  Zeid I M. Response of bean (Phaseolus vulgaris) to exogenous putrescine treatment under salinity stress[J]. Parkistan J Biol Sci, 2004,7:(5):219.
[15]  Roy P, Niyogi K, Sengupta D N, et al. Spermidine treatment to rice seedlings recovers salinity stress-induced damage of plasma membrane and PM-bound H+-ATPase in salt-tolerant and salt-sensitive rice cultivars[J]. Plant Sci, 2005,168(3):583.
[16]  Kasukabe Y, He L X, Nada K, et al. Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and upregulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana[J].Plant Cell Physiol,2004,45(6):712.
[17]  Kasukabe Y, H e L X, Watakabe Y, et al. Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase [J]. J Plant Biotechnol, 2006,23(1):75.
[18]  Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain treated bean plants protective role of exogenous polyamines [J]. Plant Sci, 2000,151(2):59.
[19]  陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:119.
[20]  郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:115.
[21]  张以顺,黄霞,陈云凤.植物生理学实验教程[M].北京: 高等教育出版社,2009:136.
[22]  Wang L J, Jiang W B, Liu H, et al. Promotion of 5-aminolevulinic acid (ALA) on germination of pakchoi (Brassica chinensis) seeds under salt stress [J]. J Integr Plant Biol,2005,47(9):1084.
[23]  Thomas J, Weinstein J D. Measurement of heme efflux and heme content in isolated developing chloroplasts [J]. Plant Physiol,1990,94:1414.
[24]  Van Huyestee R B. Porphyrin and peroxidase synthesis in cultured peanut cells [J]. Can J Bot, 1977, 55: 1340.
[25]  Thomas L P, Kraut J. The stereochemistry of peroxidase catalysis [J]. J Biol Chem,1980,255:8199.
[26]  刘晖,康琅,汪良驹.ALA对盐胁迫下西瓜种子萌发的促进效应[J].果树学报,2006,23(6):854.
[27]  宋士清,郭世荣,尚庆茂,等.外源SA对盐胁迫下黄瓜幼苗的生理效应[J].园艺学报,2006,33(1):68.
[28]  余小平,贺军民,张键,等.水杨酸对盐胁迫下黄瓜幼苗生长抑制的缓解效应[J].西北植物学报, 2002,22(2):401.
[29]  李兆亮,原永兵,刘成连,等.水杨酸对黄瓜叶片抗氧化剂酶系的调节作用[J].植物学报,1998, 40 (4):356.
[30]  Krishnamurthy R. Amelioration of salinity effect in salt tolerant rice (Oryza sativa L.) by foliar application of putrescine[J]. Plant Cell Physiol,1991,32(7):699.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133