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手掌参的化学成分

Keywords: 手参,手参属,兰科,化学成分,活性筛选

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Abstract:

目的:研究手参属药用植物手参Gymnadeniaconopsea的化学成分。方法:用乙醇提取后通过大孔树脂、硅胶、凝胶SephadexLH-20、反相闪式快速分离、中压液相色谱和高效制备液相色谱分离纯化;运用光谱数据解析鉴定化合物结构。结果:从手参药用部位块茎的乙醇提取物中分离鉴定了34个化合物,其中包括6个2-异丁基酒石酸苄酯苷:凹舌兰素A,B,E和D(1-4),dactylorhinB(5)和loroglossin(6);3个2-异丁基苹果酸苄酯苷:dactylorhinA(7),dactylorhinE(8)和militarine(9);3个木脂素类:牛蒡酚(10),lappaolA(11)和lappaolF(12);6个芳香酸(或醛或醇)衍生物酚苷类:4-β-D-吡喃葡萄糖氧基反式苯丙稀酸(13)、4-β-D-吡喃葡萄糖氧基顺式苯丙稀酸(14)、天麻素(15)、4-(β-D-吡喃葡萄糖)苯甲醛(16)、4-(β-D-吡喃葡萄糖)苄基甲基醚(17)、4-(β-D-吡喃葡萄糖)苄基乙基醚(18)和双(4-羟基苄基)醚单β-D-葡萄糖苷(19);4个环二肽:环(L-亮氨酸-L-酪胺酸)(20)、环(-亮氨酸-L-脯胺酸)(21)、环(L-缬氨酸-L-酪胺酸)(22)和环(L丙氨酸-L-苯丙胺酸)(23);1个N6-取代的腺苷类:N6-(4-羟基苄基)-腺苷(24);1个酰胺类:N-trans-阿魏酰色胺(25);9个芳香酸(或醛或醇)衍生物:3-羟基苯甲酸(26)、4-羟基异肽酸(27)、4-羟基苯甲醇(28)、4-羟基苄甲醚(29)、4-羟基苯甲醛(30)、4-羟基苯甲酸(31)、3-甲氧基-4-羟基苯甲酸(32)、反式-对羟基苯丙烯酸(33)和顺式-对羟基苯丙烯酸(34)。除化合物5~7,9,15,28~34外,其他化合物均为从手参属植物中首次分离得到;10~12对Fe2+-Cys诱导大鼠肝微粒体丙二醛(MDA)的生成有抑制作用,在1×10-6mol·L-1浓度下抑制率分别为53%,59%,52%,阳性对照药VE的抑制率为35%。

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