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桂皮醛通过下调mPGES-1和COX-2抑制IL-1β诱导的RAW264.7细胞PGE2分泌

Keywords: 桂皮醛,前列腺素E2,前列腺素合酶,环氧化酶,RAW264.7,IL-1β

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Abstract:

目的:在明确桂皮醛通过下调COX-2抑制PGE2生成的基础上,进一步探讨其抑制PGE2生成的机制。方法:采用IL-1β刺激的方法,构建炎症模型,并用ELISA法检测PGE2的分泌量,用Real-timePCR法检测mPGES-1和COX-2mRNA的表达,用Westernblotting法检测mPGES-1蛋白,以观测桂皮醛对上述指标的影响。结果:桂皮醛能显著抑制PGE2的分泌,同时,也能显著下调mPGES-1和COX-2mRNA的表达,及mPGES-1蛋白的表达。结论:桂皮醛对RAW264.7细胞PGE2生成的影响,除通过对COX-2的抑制作用外,也要归因于其对mPGES-1的抑制作用。

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