全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

鞘磷脂-胆固醇为膜材的绞股蓝总皂苷脂质体的制备及其质量评价研究

Keywords: 绞股蓝总皂苷,脂质体,胆固醇,制备工艺,正交设计

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:探讨用鞘磷脂与胆固醇制备绞股蓝总皂苷脂质体的可行性,并获得最佳制备工艺与处方。方法:以鞘磷脂和胆固醇为膜材制备绞股蓝总皂苷脂质体;脂质体以鱼精蛋白沉淀法进行包封率的测定;以包封率为评定指标,筛选该新型绞股蓝总皂苷脂质体的最佳制备方法;用正交设计优化该脂质体的制备工艺;用粒径、电位、AFM电镜表征该新型绞股蓝总皂苷脂质体。结果:用鞘磷脂和胆固醇制备绞股蓝总皂苷脂质体的最佳制备方法为乙醇注入法,通过正交设计得最佳工艺为绞股蓝总皂苷与鞘磷脂质量比为1:10,鞘磷脂与胆固醇的比例4:1,缓冲液pH7.0,水化温度45℃,包封率79.06%,粒径191.4nm,电位-33.16mV,电镜下呈规则圆形。结论:用鞘磷脂与胆固醇制备绞股蓝总皂苷脂质体方法可行,采用最佳工艺制备的绞股蓝总皂苷包封率高,形态和粒径均较好,重复性好。

References

[1]  Webb M, Logan P, Kanter P, et al. Preclinical pharmacology, toxicology and efficacy of sphingomyelin/cholesterol liposomal vincristine for therapeutic treatment of cancer[J]. Cancer Chemother Pharmacol, 1998, 42: 461.
[2]  Zant Przeworska, Stasiuk M, Gubernator J, et al. Resorcinolic lipids improve the properties of sphingomyelin-cholesterol liposomes[J]. Chem Phys Lipids, 2010,163(7): 648.
[3]  Barenholz Y. Sphingomyelin and cholesterol: from membrane biophysics and rafts to potential medical applications[J]. Subcell Biochem, 2004(37): 167.
[4]  Gabizon A, Poulos D. Liposome formulations with prolonged circulation time in blood and enhanced up take by tumors[J]. Proc Natl Acad Sci USA, 1988, 85(18): 6949.
[5]  熊欣, 顼佳音, 尹晓琴, 等. 灯盏花素不同脂质载体凝胶体外经皮渗透性评析[J]. 中国中药杂志, 2012, 37(23): 3354.
[6]  Ellens E, Morselt H, Scherphof G L. In vivo fate of large unilamellar sphingomyelin-cholesterol liposomes after intraperitoneal and intravenous injection into rats[J]. Biochim Biophys, 1981,674: 10.
[7]  Kiani M F, Yuan H, Chen X, et al. Targeting microparticles to select tissue via radiation-induced upregulation of endothelial cell adhesion molecules[J]. Pharm Res, 2002, 19: 1317.
[8]  Mora M, Saghsta M, Trombetta D, et al. Design and characterization of liposomes containing long chain N-aeyIPEs for brain delivery: penetration of liposomes incorporating GM1 into the rat brain[J]. Pharm Res, 2002, 19(10): 1430.
[9]  Cheng J C, Chung J G, Chen L D. Gypenoside induces apoptosis in human Hep3B and HA227 tumour cells[J]. Cytobios, 1999, 100(393): 37.
[10]  Chen J C, Tsai C C, Chen L D, et al. Therapeutic effect of gypenoside on chronic liver injury and fibrosis induced by CCl4 in rats[J]. Am J Chin Med, 2000, 28: 175.
[11]  Wang Q F, Chang C W, Wu C C, et al. Gypenosides induce apoptosis in human hepatoma Huh-7 cells through a calcium/reactive oxygen species-dependent mitochondrial pathway[J]. Planta Med, 2007, 73: 535.
[12]  Schild L, Chen B H, Makarov P, et al. Selective induction of apoptosis in glioma tumour cells by a Gynostemma pentaphyllum extract[J]. Tradit Chin Med, 2010, 17: 589.
[13]  Chen J C, Lu K W, Tsai M L, et al. Gypenosides induced G0/G1 arrest via CHk2 and apoptosis through endoplasmic reticulum stress and mitochondria-dependent pathways in human tongue cancer SCC-4 cells[J]. Oral Oncol, 2009,45(3):273.
[14]  Hou J, Liu S, Ma Z, et al. Effects of Gynostemma pentaphyllum makino on the immunological function of cancer patients[J]. J Tradit Chin Med, 1991, 11: 47.
[15]  Huang C, Kuo M L, Li M L, et al. Extract of Gynostemma pentaphyllum enhanced the production of antibodies and cytokines in mice[J]. Yakugaku Zasshil, 2007, 27: 889.
[16]  韩红霞, 吕世静. 三萜皂苷类化合物抗肿瘤机制的研究新进展[J]. 中国医院药学杂志, 2010, 30(1): 64.
[17]  郭海燕,莫穗林.脂质体物理稳定性和包封率的影响因素[J].中国新药杂志,2004,13(6): 498.
[18]  孙庆雪,邵伟,黄桂华.脂质体制备方法的选择[J].中成药,2010,32(8): 1397.
[19]  赵志英,谢俊,刘文-,等.羟喜树碱脂质体的制备及其大鼠体内组织分布的研究[J].中国中药杂志,2011,36(4): 450.
[20]  霍美蓉,张勇,周建平,等. N-辛基-O,N-羧甲基壳聚糖聚合物胶束对紫杉醇的增溶、缓释及其安全性初步评价[J].药学学报,2008,43(8): 855.
[21]  Dong Y, Feng S S. Methoxy poly(ethylene glycol)-poly(lactide)(MPEG-PLA) nanoparticles for controlled delivery of anticancer drugs [J]. Biomaterials, 2004, 25: 2843.
[22]  Fan Yu, Chenghua He, Ayman Y Waddad, et al. N-octyl-N-arginine-chitosan(OACS)micelles for gambogic acid oral delivery: preparation, characterization and its study on in situ intestinal perfusion[J]. Drug Dev Ind Pharm, DOI: 10.3109/03639045.2013.786723.
[23]  David A Mannock, Ruthven N A H Lewis, Todd P W McMullen, et al. The effect of variations in phospholipid and sterol structure on the nature of lipid-sterol interactions in lipid bilayer model membranes[J]. Chem Phys Lipids, 2010, 163: 403.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133