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人源肠内菌转化通脉方的化学研究

Keywords: 通脉方,人源肠内菌,异黄酮,葛根素,大豆苷元,“前药”

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Abstract:

研究人源肠内菌转化"通脉方"的化学成分。采用硅胶、SephadexLH-20、半制备性高效液相等柱色谱和重结晶等方法进行分离纯化,根据MS和NMR等谱学数据鉴定化合物的结构。分离鉴定了26个化合物,分别为苯丙酸(1)、6"-O-乙酰基葛根素(2)、芒柄花素(3)、大豆苷元(4)、对羟基苯丙酸(5)、吲哚-3-丙酸(6)、染料木素(7)、异芒柄花素(8)、异芒柄花苷(9)、(-)-野葛醇B-2"-O-吡喃葡萄糖苷(10a)和(+)-野葛醇B-2"-O-吡喃葡萄糖苷(10b)混合物、8-羟基大豆苷元(11)、野葛醇A(12)、3'-甲氧基-6"-O-乙酰基葛根素(13)、6"-O-乙酰基大豆苷(14)、3'-甲氧基大豆苷(15)、野葛醇B(16)、3-甲基尿嘧啶(17)、染料木苷(18)、大豆苷(19)、3'-甲氧基葛根素(20)、葛根素芹菜糖苷(21)、獐芽菜苦苷(22)、大豆苷元-7,4'-O-双葡萄糖苷(23)、腺嘌呤(24)、3'-羟基葛根素(25)和葛根素(26)。"通脉方"经人源肠内菌生物转化,所含苷类化合物被转化为苷元和/或少糖基化合物,亦包括羟基化反应和脱甲基反应;苷类化合物是"前药"。

References

[1]  Yang X W,Wang N,Li W,et al. Biotransformation of 4,5-O-dicaffeoylquinic acid methyl ester by human intestinal flora and evaluation on their inhibition of NO production and antioxidant activity of the products [J]. Food Chem Toxicol,2013,55:297.
[2]  Tian X J,Yang X W,Yang X D,et al. Studies of intestinal permeability of 36 flavonoids using Caco-2 cell monolayer model [J]. Int J Pharm,2009,367(1):58.
[3]  李长彪,赵秀红,刘长江,等.大豆异黄酮糖苷及苷元调节免疫功能的比较研究 [J].食品科学,2007,28(5):320.
[4]  Burda S,Oleszek W. Antioxidant and antiradical activities of flavonoids [J]. J Agric Food Chem,2001,49:2774.
[5]  吴婷婷,崔桂友,颜云荞,等. 8-羟基大豆苷元的生物活性研究进展 [J].食品与发酵工业,2012,38(2):141.
[6]  Esaki H,Kawakishi S,Morimitsu Y,et al. New potent antioxidative o-dihydroxyisoflavones in fermented Japanese soybean products [J]. Biosci Biotechnol Biochem,1999,63(9):1637.
[7]  Park J S,Kim D H,Lee J K,et al. Natural ortho-dihydroxyisoflavone derivatives from aged Korean fermented soybean paste as potent tyrosinase and melanin formation inhibitors [J]. Bioorg Med Chem Lett,2010,20(3):1162.
[8]  Chen Y C,Inaba M,Abe N,et al. Antimutagenic activity of 8-hydroxyisoflavones and 6-hydroxydaiazein from soybean miso [J]. Biosci Biotechnol Biochem,2003,67(4):903.
[9]  Fujita T,Funako T,Hayashi H. 8-Hydroxydaidzein,an aldose reductase inhibitor from okara fermented with Aspergillus sp. HK-388 [J]. Biosci Biotechnol Biochem,2004,68(7):1588.
[10]  Kiriakidis S,Hogemeier O,Starcke S,et al. Novel tempeh(fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay [J]. Brit J Nutr,2005,93(3):317.
[11]  Jin S E,Son Y K,Min B S,et al. Anti-inflammatory and antioxidant activities of constituents isolated from Pueraria lobata roots [J]. Arch Pharm Res,2012,35(5):823.
[12]  卫生部. 中华人民共和国卫生部药品标 准·中 药成方制剂. 第4册 [S]. 1991: 169.
[13]  卫生部. 中华人民共和国卫生部药品标 准·中 药成方制剂. 第20册 [S]. 1991: 302.
[14]  王付荣,葛喜珍,杨秀伟.通脉方化学成分 [J].中国实验方剂学杂志,2011,17(20): 61.
[15]  Wang F R,Yang X W,Zhang Y,et al. Three new isoflavone glycosides from Tongmai granules [J]. J Asian Nat Prod Res,2011,13(4): 319.
[16]  杨秀伟. 中药成分的肠内和肝内行为与中药有效成分的确定 [J]. 中草药,2004(增刊):1.
[17]  杨秀伟.中草药化学成分的研究 [J].中草药,2007,38(7):961.
[18]  杨秀伟,徐嵬.中药化学成分的人肠内细菌生物转化模型和标准操作规程的建立 [J].中国中药杂志,2011,36(1):19.
[19]  吴霞,于志斌,叶蕴华,等.荜茇化学成分的研究(I) [J].中草药,2008,39(2):178.
[20]  Ding Y G,Zhang D W,Liu S M,et al. Selective acetylation of puerarin by Rhodococcus sp. [J]. J Chin Pharm Sci,2012,21(3):269.
[21]  邵建华,张玉伟,王金辉,等.蓬子菜的化学成分[J].中国药物化学杂志,2009,19(4): 288.
[22]  Matsuda K,Toyoda H,Nishio H,et al. Control of the bacterial wilt of tomato plants by a derivative of 3-indolepropionic acid based on selective actions on Ralstonia solanacearum [J]. J Agric Food Chem,1998,46(10):4416.
[23]  王凤芹,蒋可志,李祖光.银杏叶提取物中染料木素的分离纯化及结构鉴定[J].色谱,2007,25(4):509.
[24]  Zhang D W,Dai S J,Liu W H,et al. Chemical constituents from the vines of Pueraria lobata [J]. Chin J Nat Med,2010,8(3):196.
[25]  郑尚珍,康淑荷,沈彤. 木香薷化学成分的研究[J]. 西北师范大学学报:自然科学版,2000,36(1):51.
[26]  Nohara T,Kinjo J,Furusawa J,et al. But-2-enolides from Pueraria lobata and revised structures of puerosides A,B and sophoroside A[J]. Phytochemistry,1993,33(5):1207.
[27]  Nechepurenko Ⅳ,Polovinka M P,Komarova N I,et al. Low-molecular-weight phenolic compounds from Hedysarum theinum roots [J]. Chem Nat Compd,2008,44(1):31.
[28]  Bai B,Fu Y C,Si X G. Plant extracts and compositions for reducing blood lipid levels: US,2008/0260877 A1[P]. 2008-10-23.
[29]  张德武,任燕,戴胜军,等.野葛藤中的异黄酮类化合物 [J].中国中药杂志,2009,34(24):3217.
[30]  Choi Y H,Hong S S,Shin Y S,et al. Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity [J]. Arch Pharm Res,2010,33(10):1651.
[31]  Wong J L,Fuch D S. Competitive isomerization and dealkylation of 2,4-dialkoxypyrimidines in aqueous and nonaqueous medial [J]. J Org Chem,1970,36(11):3786.
[32]  Zhou Y,Di Y T,Gesang S L,et al. Secoiridoid glycosides from Swertia mileensis [J].Helv Chim Acta,2006,89(1):94.
[33]  陈泉,吴立军,阮丽军.中药淡竹叶的化学成分研究(Ⅱ) [J].沈阳药科大学学报,2002,19(4):257.
[34]  杨秀伟. 中药成分的吸收、分布、代谢、排泄、毒性与药效 [M]. 北京: 中国医药科技出版社,2006.
[35]  Wang R F,Yuan M,Yang X B,et al. Intestinal bacterial transformation——a nonnegligible part of Chinese medicine research [J]. J Asian Nat Prod Res,2013,15(5):532.
[36]  杨秀伟.基于体内过程的中药有效成分和有效效应物质的发现策略 [J].中国中药杂志,2007,32(5):365.

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