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银杏叶提取物对泪腺腺样囊性癌ACC-2细胞增殖及Survivin,TIP30基因表达的影响

Keywords: 银杏叶提取物,ACC-2细胞,Survivin基因,TIP30基因

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Abstract:

探讨银杏叶提取物(EGB)对泪腺腺样囊性癌ACC-2细胞增殖、凋亡的影响,并从基因和蛋白水平上分析EGB对Survivin和TIP30基因表达的影响。不同浓度EGB处理体外培养的人泪腺腺样囊性癌ACC-2细胞,应用MTT法检测细胞增殖;AnnexinV/PI双染色流式细胞仪检测细胞凋亡和细胞周期;RT-PCR和Westernblotting分析Survivin,TIP30基因和蛋白的表达。结果发现EGB对ACC-2细胞的体外增殖具有抑制作用,量效关系显著,与对照组比较有统计学差异(P<0.01),半数抑制浓度(IC50)为88mg·L。经流式细胞仪检测表明,EGB能使ACC-2细胞G0/G1期逐渐增加,G2/M期和S期逐渐减少,并且随着剂量的增加,ACC-2细胞凋亡率明显增加(P<0.05或P<0.01)。RT-PCR与Western杂交结果一致表明随着EGB浓度的升高,Survivin基因表达明显减少(P<0.01),而TIP30基因表达则显著提高(P<0.01)。因此,EGB能有效抑制人泪腺腺样囊性癌ACC-2细胞Survivin基因表达,并促进TIP30的表达,诱导ACC-2细胞凋亡,从而抑制肿瘤细胞增殖。该研究为中药成分在肿瘤治疗中的应用提供了一定的理论和实验依据。

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