全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

高效液相质谱联用技术鉴定刺五加注射液中苯丙素类化学成分

Keywords: 刺五加注射液,LC-MSn,LC-Q-TOF-MS,苯丙素类成分

Full-Text   Cite this paper   Add to My Lib

Abstract:

刺五加药材的化学组成已有报道,而未见刺五加注射液化学物质基础的研究报道。该研究采用液相色谱离子阱质谱(LC-MSn)和液相色谱四极杆飞行时间质谱(LC-Q-TOF-MS)技术对刺五加注射液中苯丙素类化学成分进行定性分析。采用UltmateTMXB-C18色谱柱(4.6mm×250mm,5μm),以0.5%甲酸溶液和乙腈为流动相进行梯度洗脱,流速0.8mL·min-1,柱温20℃。根据高分辨和多级质谱数据,并结合对照品及文献数据,在刺五加注射液中共推断出54个苯丙素类成分,包括苯丙酸类成分34个,木脂素类成分16个,香豆素类成分4个,其中28个成分在刺五加中未见报道,通过对照品比对鉴定了14个化合物结构。该研究建立的方法可以简便快速地对刺五加注射液中的苯丙素类化学成分进行鉴定,研究结果为明确刺五加注射液化学物质基础提供了科学数据。

References

[1]  Ishimaru K, Sudo H, Satake M, et al. Phenyl glucosides from a hairy root culture of Swertia japonica[J]. Phytochemistry, 1990, 29(12): 3823.
[2]  蒋礼. 刺五加化学成分及其生物活性探讨 . 延边:延边大学, 2010.
[3]  张涛, 朴俊虹, 袁蕾, 等. 刺五加化学成分及自由基清除活性研究[J]. 中草药, 2012(6): 1057.
[4]  Ida Y, Satih Y, Ohtsuka M, et al. Phenolic constituents of Pheliodendron amurense bark[J]. Phytochemistry, 1994, 35(1): 209.
[5]  Nishibe S, Kinoshita H, Takeda H, et al. Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats[J]. Chem Pharm Bull, 1990, 38(6): 1763.
[6]  Li Z F, Wu Z H, Chen G, et al. Two new compounds from Acanthopanax senticosus Harms[J]. J Asian Nat Prod Res, 2009, 11: 715.
[7]  刺五加注射液质量标准草案[S]. 2011-11-24.
[8]  Li F, Li W, Fu H, et al. Pancreatic lipase-inhibiting triterpenoid saponins from fruits of Acanthopanax senticosus[J]. Chem Pharm Bull, 2007, 55: 1087.
[9]  陈貌连, 宋凤瑞, 郭明全, 等. 刺五加叶中黄酮类化合物的结构鉴定[J]. 高等学校化学学报, 2002(5): 805.
[10]  Zhang X Q, Lai Y C, Wang L, et al. Phenylpropanoid constituents from Acanthopanax senticosus[J]. Biochem Syst Ecol, 2011, 39: 861.
[11]  刘建娣,梁琼麟,罗国安,等.刺五加注射液指纹图谱的建立及其在质量控制中的应用[J].中成药,2006,28(1): 3.
[12]  于风平,连传宝.超快速液相色谱法测定刺五加注射液中紫丁香苷和绿原酸的含量[J].中国药师,2010,13(12): 1769.
[13]  焦正花,顾秀琰,杨小源,等. RP-HPLC法测定刺五加注射液中紫丁香苷、紫丁香树脂苷的含量[J].中国中医药信息杂志,2007,14(8): 50.
[14]  金佩芬. 高效液相色谱法测定刺五加注射液中异秦皮啶含量 [J]. 中国药业, 2009, 18(3): 9.
[15]  Clifford M N, Johnston K L, Knight S, et al. Hierarchical scheme for LC-MSn identification of chlorogenic acids[J]. J Agric Food Chem, 2003, 51(10): 2900.
[16]  王放,张加余,王青,等. HPLC-HR-MSn法鉴定苦碟子注射液中酚酸类成分[J].中南药学,2013,11(8): 561.
[17]  Zhang Y, Zhao Q, Ma J, et al. Chemical characterization of phenolic compounds in erigeron injection by rapid-resolution LC coupled with multi-stage and quadrupole-TOF-MS[J]. Chromatographia, 2010, 72(7/8): 651.
[18]  Clifford M N, Khight S, Kuhnert N. Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn[J]. J Agric Food Chem, 2005, 53(10): 3821.
[19]  Matei M F, Jaiswal R, Kuhnert N. Investigating the chemical changes of chlorogenic acids during coffee brewing: conjugate addition of water to the olefinic moiety of chlorogenic acids and their quinides [J]. J Agric Food Chem, 2012, 60(49): 12105.
[20]  Auriol D, Nalin R, Robe P, et al. Water soluble and activable phenolics derivatives with dermocosmetic and therapeutic applications and process for preparing said derivatives:America,us 20110183930A1 . 2011-07-28.
[21]  Kashiwada Y, Ahmed F A, Kurimoto S I, et al. New alpha-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam[J]. J Nat Med, 2012, 66(1): 217.
[22]  Yang G E, Li W, Huang C, et al. Phenolic constituents from the stems of Acanthopanax senticosus [J]. Chem Nat Compd, 2011, 46(6): 876.
[23]  Mcnamara C E, Perry N B, Douglas J A, et al. A new glucosyl feruloyl quinic acid as a potential marker for roots and rhizomes of goldenseal, Hydrastis canadensis[J]. J Nat Prod, 2004, 67(11): 1818.
[24]  Daneshtalab M, Jin C, Micetich R G. Phenylpropanoid glycosides from Stellera chamaejasme[J]. Phytochemistry, 1999, 50(4): 677.
[25]  Park B J, Tomohiko M. Feruloyl, caffeoyl, and flavonol glucosides from Equisetum hyemale[J]. Chem Nat Compd, 2011, 47(3): 363.
[26]  隆颖,栗建明,莫结丽,等. HPLC法测定刺五加注射液中紫丁香苷和异嗪皮啶的含量[J].中国药品标准,2011,12(6): 410.
[27]  孙国祥, 于秀明, 王佳庆. 刺五加注射液HPLC数字化指纹图谱研究[J]. 药物分析杂志, 2009, 29(3): 356.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133