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PEG修饰β-谷甾醇制备新型绞股蓝总皂苷长循环脂质体的研究

Keywords: 绞股蓝总皂苷,长循环脂质体,β-谷甾醇,聚乙二醇

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Abstract:

目的:探讨用聚乙二醇(PEG)修饰β-谷甾醇(PEG-Sito)制备新型绞股蓝总皂苷长循环脂质体的可行性。方法:以丁二酸酐为连接臂连接β-谷甾醇与PEG2000合成PEG-Sito;以鞘磷脂和PEG-Sito为膜材采用乙醇注入法制备绞股蓝总皂苷长循环脂质体;脂质体以鱼精蛋白沉淀法进行包封率的测定;1H-NMR验证PEG-Sito的合成;粒径、电位、AFM电镜表征该新型绞股蓝总皂苷长循环脂质体。结果:1H-NMR证实了PEG-Sito的合成成功,采用乙醇注入法用PEG-Sito为材料制备长循环脂质体的包封率为74.3%,粒径288.1nm,电位-20.25mV,电镜下呈规则圆形。结论:用PEG-Sito为材料制备绞股蓝总皂苷长循环脂质体方法可行,绞股蓝总皂苷包封率高,形态和粒径均较好。

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