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LC-Q-TOF-MS及LC-IT-MS分析酸枣仁汤的化学成分

Keywords: LC-Q-TOF-MS,LC-IT-MS,酸枣仁汤,化学成分

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Abstract:

采用液相色谱-四级杆-飞行时间质谱(LC-Q-TOF-MS)及液相色谱-离子阱质谱(LC-IT-MS)系统剖析了酸枣仁汤中化学成分。色谱柱为AgilentZorbaxSB-C18,RapidResolutionHT(4.6mm×50mm,1.8μm);以乙腈(A)-0.05%甲酸溶液(B)为流动相,梯度洗脱;流速0.6mL·min-1;柱温30℃。共鉴定了酸枣仁汤中48个化合物,并对化合物的药材来源进行了归属,首次报道了方剂中的31个化合物。LC-Q-TOF-MS结合LC-IT-MS可以简便、快速地对酸枣仁汤中化学成分定性分析,本研究结果为酸枣仁汤质量控制和药效物质基础研究提供了一定的理论依据。

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