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松果菊苷对乙肝病毒复制和抗原表达的影响

Keywords: 松果菊苷,乙肝病毒,HepG2.2.15细胞,转基因小鼠

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Abstract:

以HBV基因转染的HepG2.2.15细胞为体外模型,研究松果菊苷对乙肝病毒(HBV)复制和抗原表达的影响,采用ELISA法测定细胞上清乙肝表面抗原(HBsAg)和E抗原(HBeAg)水平;采用荧光定量PCR法检测HBVDNA水平。以HBV转基因小鼠为体内模型,研究松果菊苷对HBV复制的影响,首先采用荧光定量PCR法检测转基因小鼠肝组织HBVDNA水平,采用ELISA法检测小鼠血清HBsAg和HBeAg水平;同时检测血清转氨酶水平和肝组织病理变化;然后将HBV表达阳性的转基因小鼠随机分为5组:对照组、拉米夫定(50mg·kg-1)组和松果菊苷高、中、低剂量(50,25,12.5mg·kg-1)组。每天灌胃给药1次,连续给药30d。第31天分离小鼠血清检测HBVDNA,HBsAg和HBeAg水平;提取肝组织DNA测定其HBVDNA水平,同时作肝组织病理检查。结果表明:松果菊苷在50,100mg·L-1作用HepG2.2.15细胞6d后能显著抑制HBsAg和HBeAg表达,其最高抑制率分别为42.68%,46.29%;同时在100mg·L-1对HBVDNA具有抑制作用。此外,松果菊苷在50mg·kg-1剂量时能显著抑制HBV转基因小鼠HBsAg与HBeAg表达,其抑制率分别为42.82%,29.12%,同时对转基因小鼠血清HBVDNA水平有显著抑制作用。提示松果菊苷对HBV复制和抗原表达具有较强抑制作用。

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