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牛白藤的化学成分及其免疫抑制活性研究

Keywords: 牛白藤,(6S,9S)-vomifoliol,环烯醚萜,三萜,甾体,免疫抑制活性

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Abstract:

研究茜草科耳草属植物牛白藤Hedyotishedyotidea(DC.)Merr.干燥藤茎的化学成分及免疫抑制活性,为进一步开发利用牛白藤提供依据。以硅胶、凝胶柱色谱、制备高效液相色谱进行分离纯化,并应用MS和NMR技术分析鉴定化合物结构,分离得到11个化合物吐叶醇(6S,9S-vomifoliol,1),桦木酮酸(betulonicacid,2),白桦脂酸(betulinicacid,3),白桦醇(betulin,4),表白桦脂酸(3-epi-betulinicacid,5),乌苏酸(ursolicacid,6),β-谷甾醇(β-sitosterol,7),stigmast-4-en-3-one(8),7β-hydroxysitosterol(9),(3β,7β)-7-methoxystigmast-5-en-3-ol(10)和巴戟醚萜(morindacin,11)。化合物1,2,4,8~11均首次从该植物中分得,化合物1,2,8~11均首次从耳草属植物中分得,化合物9,10均首次从茜草科植物中分得。通过体外淋巴细胞转化实验测试化学成分的免疫抑制活性,化合物4,6,9均有较强的免疫抑制活性。

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