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基于UPLC-Q-TOF-MS比较雷公藤与昆明山海棠叶片的代谢产物差异

Keywords: UPLC-Q-TOF-MS,雷公藤,昆明山海棠,叶片,差异代谢物

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Abstract:

为了区分雷公藤与昆明山海棠之间的代谢产物差异,采用UPLC-Q-TOF-MS对采自5个地区的雷公藤与昆明山海棠叶片代谢物进行分析,通过系统聚类、主成分分析研究雷公藤与昆明山海棠叶片代产物特征,利用OPLS-DA模型进一步分析,挑选出VIP>5.0,r>0.85的差异代谢物,再对差异代谢物进行mann-Whitney非参数检验,将满足P<0.001,ROC曲线下面积AUC=1的化合物确定为区分雷公藤与昆明山海棠的潜在生物标记物.结果表明,雷公藤与昆明山海棠叶片代谢物差异显著,分析次生代谢物信息,雷公藤与昆明山海棠可分为二类,同时筛选获得区分二者的潜在生物标记物23个.

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