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复方银杏叶制剂对非酒精性脂肪肝的干预作用及机制

Keywords: 非酒精性脂肪肝,肠道紧密连接蛋白,内毒素,复方银杏叶制剂

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Abstract:

目的:探究复方银杏叶制剂(CGB)对非酒精性脂肪肝(NAFLD)的干预作用机制。方法:采用高脂饮食诱导C57BL/6小鼠NAFLD模型,于造模第2周起以600,200mg·kg-1·d-1的CGB灌胃8周,观察小鼠血清生化指标谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、胆固醇(CHOL)、血清内毒素(LPS)水平、肝组织病理变化与肝脏TNF-α表达;电镜观察小肠上皮细胞紧密连接变化,测定ZO-1,Occludin,Claudin-1紧密连接蛋白表达。结果:与正常组比较,模型组小鼠肝脏发生明显脂肪变性,TNF-α表达显著增高(P<0.01);血清ALT,AST,TG,CHOL与LPS水平均显著增高(P<0.01,P<0.05);肠上皮紧密连接结构受损,ZO-1,Occludin,Claudin-1表达显著降低(P<0.01)。与模型组比较,CGB高、低剂量组明显减轻肝组织脂肪变性,TNF-α表达显著降低(P<0.01,P<0.05);血清AST,TG,CHOL与LPS水平均显著降低(P<0.01,P<0.05);肠道紧密连接破坏程度减轻,ZO-1,Occludin表达显著增高(P<0.05)。结论:CGB可以保护肠道紧密连接,减少LPS肠渗漏,缓解肝脏脂肪变性和炎症,防治NAFLD的发生发展。

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