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华中枸骨黄烷类成分体外抗炎与自由基清除活性

Keywords: 华中枸骨,黄烷,抗炎,自由基清除

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Abstract:

为研究华中枸骨中多个黄烷类成分(1~6)体外抗炎与自由基清除活性,并初步分析其构效关系。采用脂多糖诱导小鼠RAW264.7巨噬细胞炎症模型,MTT法测定细胞毒性作用,Griess法测定NO生成量,ELISA法检测细胞上清液中TNF-α,IL-1β,IL-6,PGE2的含量;体外测定清除DPPH自由基,超氧阴离子自由基和羟自由基活性。结果表明黄烷1,3,4,6体外显示很强的抗炎活性,能显著抑制NO,TNF-α,IL-1β和IL-6的分泌,化合物1的抑制作用最强,黄烷2和5显示相对较弱的抑制活性,体外自由基清除活性顺序与抗炎活性相似。因此,黄烷1,3,4,6是华中枸骨抗炎和自由基清除活性的活性成分,构效关系初步分析表明4'-羟基为黄烷类化合物显示抗炎和自由基清除活性的主要结构部位。

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