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桂枝茯苓胶囊及其活性成分组合物抗炎作用与机制研究

Keywords: 桂枝茯苓胶囊,脂多糖,抗炎

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Abstract:

该研究旨在脂多糖(LPS)刺激RAW264.7细胞模型上,对桂枝茯苓胶囊及活性成分组合物(15个成分组成)进行体外抗炎活性量效评价,并对其作用机制进行初步探索。采用MTT法检测桂枝茯苓胶囊及活性成分组合物对RAW264.7细胞活力的影响,ELISA法检测桂枝茯苓胶囊及活性成分组合物对LPS诱导RAW264.7细胞释放IL-1β,TNF-α和PGE2的影响,Westernblot法检测桂枝茯苓胶囊及活性成分组合物对IL-1β和mPGES-1表达的影响。结果显示在测定浓度下,桂枝茯苓胶囊及活性成分组合物对RAW264.7细胞活力无显著影响;ELISA检测结果表明桂枝茯苓胶囊及活性成分组合物能有效抑制IL-1β,TNF-α和PGE2的释放,且均呈现一定的浓度-效应依赖关系;Westernblot结果表明桂枝茯苓胶囊及活性成分组合物能有效降低IL-1β与mPGES-1的表达。以上结果表明桂枝茯苓胶囊及活性成分组合物能有效抑制LPS诱导的炎症因子释放,其作用机制可能与降低IL-1β与mPGES-1的表达有关。该研究为阐明桂枝茯苓胶囊的抗炎活性和相关机制奠定了实验基础,同时对桂枝茯苓胶囊的效应物质基础解析提供了一定的理论依据。

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