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雷公藤牻牛儿基牻牛儿基焦磷酸合酶基因全长cDNA的获得及生物信息学分析

Keywords: 雷公藤,牻牛儿基牻牛儿基焦磷酸合酶,克隆,生物信息学分析

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Abstract:

该研究利用cDNA末端快速扩增(RACE)技术从雷公藤悬浮细胞中克隆得到一条GGPPS全长cDNA(GeneBank:KM978333),并对其进行多重序列比对、蛋白结构预测及构建系统进化树等生物信息学分析。所克隆的TwGGPPScDNA全长1857bp,编码含514个氨基酸的蛋白序列,相对分子质量为57.13kDa,等电点为7.85,具有5个保守域和2个功能域,亚细胞定位预测显示其可能位于质膜上,多重序列比对及系统进化树结果显示与其他植物GGPPS家族具有较高同源性。实验首次克隆了雷公藤GGPPS基因,为进一步研究雷公藤甲素等二萜化合物生物合成途径奠定基础。

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