全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

亲和色谱应用于天然活性成分筛选的研究进展

Keywords: 亲和色谱,筛选,天然药物,细胞膜色谱,脂筏色谱

Full-Text   Cite this paper   Add to My Lib

Abstract:

亲和色谱法(affinitychromatography)是一种利用固定相的结合特性来分离分子的色谱方法。利用化合物和固定相表面之间的某种特异性亲和力,将目标化合物从大量无亲和活性的化合物中分离出来。该方法可同时进行色谱分离和活性筛选,作为一种高通量筛选方法广泛应用于各种活性药物的筛选。该文主要针对亲和色谱的发展历史、筛选技术类型及其在天然活性成分筛选中的应用进行了综述。并就其应用前景进行讨论,以期更好地发挥亲和色谱在药物筛选中的应用。

References

[1]  Li Y F, Chen Y, Xiao C Y, et al. Rapid screening and identification of α-amylase inhibitors from Garcinia xanthochymus using enzyme-immobilized magnetic nanoparticles coupled with HPLC and MS[J].J Chromatogr B, 2014, 960 (1):166.
[2]  Zhang A Z, Ye F G, Lu J Y, et al. Screening α-glucosidase inhibitor from natural products by capillary electrophoresis with immobilised enzyme onto polymer monolith modified by gold nanoparticles[J].Food Chem, 2013, 141(3): 1854.
[3]  Jiang T F, Liang T T, Wang Y H, et al. Immobilized capillary tyrosinase microreactor for inhibitor screening in natural extracts by capillary electrophoresis[J]. J Pharm Biomed Anal, 2013, 84: 36.
[4]  Min W A, Cui S M, Wang W P, et al. Capillary electrophoresis applied to screening of trypsin inhibitors using microreactor with trypsin immobilized by glutaraldehyde[J]. Anal Biochem, 2013, 438(1): 32.
[5]  Lopes Vilela A F, Silva J I, Curcino Vieira L C, et al. Immobilized cholinesterases capillary reactors on-flow screening of selective inhibitors[J].J Chromatogr B, 2013, 968, (1): 87.
[6]  黄燕, 田清清, 李谭瑶, 等. 亲和色谱法筛选中药中血管紧张素转化酶抑制剂[J]. 中国科学, 2009, 38(8): 760.
[7]  王芳焕, 龚波林.人血清白蛋白生物色谱柱的性能研究及应用于铁棒锤中活性成分的筛选[J]. 化学通报, 2010(9):819.
[8]  Hadjmohammadi M, Salary M. Biopartitioning micellar chromatography with sodium dodecyl sulfate as a pseudo α1-acid glycoprotein to the prediction of protein-drug binding[J]. J Chromatogr B, 2013, 912(1):50.
[9]  Vuignier K, Guillarme D, Veuthey J L, et al. High performance affinity chromatography (HPAC) as a high-throughput screening tool in drug discovery to study drug-plasma protein interactions[J]. J Pharm Biomed Anal, 2013, 74 (3) :205.
[10]  Zhou C L, Dong Y M, Li Z Y, et al. Electrochemistry of magnolol and interaction with DNA[J]. J Electroanal Chem, 2010, 642.
[11]  Su X Y, Hu L H, Kong L, et al. Affinity chromatography with immobilized DNA stationary pHSAe for biological fingerprinting analysis of traditional Chinese medicines[J]. J Chromatogr A, 2007, 1154:132.
[12]  方艺霖, 张艺, 肖小河, 等. 细胞膜色谱技术用于中药活性成分筛选的研究进展[J].中草药, 2008, 39(7):3.
[13]  Wang C H, He L C, Wang N, et al. Screening anti-inflammatory components from Chinese traditiona medicines using a peritoneal macrophage/cell membrane chromatography-offline-GC/MS method[J]. J Chromatogr B, 2009, 877: 3019.
[14]  Liu J, Wang S C, Sun J Y, et al. Screening of osteoanagenesis-active compounds from Scutellaria baicalensis Georgi by hPDLC/CMC-online-HPLC/MS[J]. Fitoterapia, 2014, 93: 105.
[15]  Chen X F, Cao Y, Lv D Y, et al. Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines[J]. J Chromatogr A, 2012, 1242: 67.
[16]  王嗣岑, 孙萌, 张彦民, 等. 用高表达EGFR 细胞膜色谱-HPLC/MS 联用快速筛选独活中抗EGFR 活性成分[J].中国科学, 2010, 40: 750.
[17]  Sun M, Ren J, Du H, et al. A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR[J]. J Chromatogr B, 2010, 878(28): 2712.
[18]  Li M, Wang S C, Zhang Y M, et al. An online coupled cell membrane chromatography with LC/MS method for screening compounds from Aconitum carmichaeli Debx. acting on VEGFR-2 [J]. J Pharm Biomed Anal, 2010, 53(4): 1063.
[19]  Clonis Y D. Affinity chromatography matures as bioinformatic and combinatorial tools develop[J]. J Chromatogr A, 2006, 1101(1/2): 1.
[20]  于世林. 亲和色谱方法及应用[M]. 北京: 化学工业出版社, 2008: 6.
[21]  王序, 韩桂秋, 李荣芷,等. 现代生物分析法对常用中药的筛选研究[J]. 北京医科大学学报, 1986, 18(1): 31.
[22]  Wang S X, Zhao K, Zang W J, et al. Highly selective screening of the bioactive compounds in Huoxue capsule using immobilized β2-adrenoceptor affinity chromatography[J]. Anal Biochem, 2014, 457(15) : 1.
[23]  郑晓晖, 赵新锋, 杨荣, 等. β2-肾上腺素受体亲和色谱及其在苦杏仁活性成分筛选中的应用[J]. 科学通报, 2007, 52(18): 2111.
[24]  Calleri E, Fracchiolla G, Montanari R, et al. Frontal affinity chromatography with MS detection of the ligand binding domain of PPARγ receptor: ligand affinity screening and stereoselective ligand-macromolecule interaction[J]. J Chromatogr A, 2012,1232(6): 84.
[25]  刘倩, 寇俊萍, 黄娅琳, 等. 以人参皂苷 Rg1为配基的亲和色谱介质的制备与初步应用[J]. 中国药科大学学报, 2010, 41(5): 451.
[26]  Ding X, Chen X F, Cao Y, et al. Quality improvements of cell membrane chromatographic column[J]. J Chromatogr A, 2014, 1359: 330.
[27]  Hanzal Bayer M F, Hancock J F. Lipid rafts and membrane traffic[J]. FEBS Lett, 2007, 581: 2098.
[28]  Tong S S, Fu M, Cao X, et al. Lipid raft stationary PHSAe chromatography for screening anti-tumor components from Galla chinensis [J]. Chromatographia, 2014, 77:419.
[29]  Madepalli K, Lakshmana, Subhojit R, et al. Amyloidogenic processing of APP in lipid rafts[J]. Open Biol J, 2010, 3: 21.
[30]  Fabelo N, Martín V, Gabriel S, et al. Severe alterations in lipid composition of Frontal Cortex lipid rafts from parkinson\'s disease and incidental parkinson\'s disease[J]. Mol Med, 2011, 17 (9/10) : 1107.
[31]  Gonzalez Munoz E, Lopez Iglesias C, Calvo M. Caveolin-1 loss of function accelerates glucose transporter 4 and insulin receptor degradation in 3T3-L1 adipocytes[J]. Endocrinology, 2009, 150(8): 3493.
[32]  谢建春, 朱丽荔, 徐筱杰. 分子烙印亲和色谱与质谱联用实现中草药活性成分分离鉴定一体化[J]. 化学学报, 2002, 60(3): 385.
[33]  Huang M X, Pang W S, Zhang J, et al. A target analogue imprinted polymer for the recognition of antiplatelet active ingredients in Radix Salviae Miltiorrhizae by LC-MS-MS[J]. J Pharmaceut Biomed, 2012, 58: 12.
[34]  袁小红, 徐筱杰, 朱伟. 柯里拉京分子印迹聚合物固相萃取叶下珠中的柯里拉京及结构类似物[J]. 中国药学杂志, 2010, 45(8): 574.
[35]  肖淑娟, 李红霞, 于守武. 分子印迹固相萃取法提取花生壳中木犀草素[J]. 化工进展, 2010, 29(2): 293.
[36]  赵强, 乐晓春, 核酸适配体亲和色谱的研究进展[J].色谱, 2009, 27(5):556.
[37]  陈亮, 陈婷, 徐强, 免疫亲和色谱特异性剔除中药方剂四逆散中的柚皮苷[J].色谱, 2006, 24(3): 243.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133