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牛蒡子苷元对自发性糖尿病GK大鼠合并高血压大血管病变的保护作用

Keywords: GK大鼠,胸主动脉,血压,白细胞计数,血小板参数,血管内皮生长因子,牛蒡子苷元

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Abstract:

探讨牛蒡子苷元对自发性糖尿病(goto-kakizaki,GK)大鼠合并高血压大血管病变的保护作用。将6周龄GK大鼠按血糖水平随机分为模型组,牛蒡子苷元低、中、高剂量组(12.5,25,50mg·kg-1),以Wistar大鼠作为正常组。所有GK大鼠饲喂高糖高脂饲料,16周后灌胃10mg·kg-1·d-1L-N-硝基精氨酸甲酯(L-NAME)连续8周。造模同时各组动物每天上下午灌胃给药2次,模型组和正常组灌溶剂。实验开始、中期及结束期检测血糖,实验结束前检测血压,麻醉后腹主动脉采血检测血常规,放血后取胸主动脉固定制作石蜡切片,观察形态,免疫组化方法检测血管内皮生长因子(VEGF)表达。结果显示,所有GK大鼠血糖持续升高,给药组与模型组相比没有明显差别,实验结束时GK大鼠血压显著升高,牛蒡子苷元可以明显降低GK大鼠血压,且具有剂量依赖性。血常规检测结果显示GK大鼠白细胞总数及分类计数均升高,血小板参数异常,PLT减少,MPV,PDW增高,牛蒡子苷元可以显著降低白细胞总数和分类计数,降低MPV,PDW。胸主动脉形态学观察发现GK大鼠血管内膜病变明显,牛蒡子苷元可以减轻病变程度,VEGF免疫组化染色结果表明模型组GK大鼠VEGF表达较多,牛蒡子苷元各剂量组表达较少。结果表明牛蒡子苷元对GK大鼠大血管具有保护作用,机制可能与降低血压、抗炎、改善血小板功能、减少VEGF表达有关。

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