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高效液相色谱法测定土茯苓药材中7种活性成分的含量

Keywords: 土茯苓,(-)-表儿茶素,5-O-咖啡酰基莽草酸,二氢黄酮,肖菝葜,高效液相色谱法

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Abstract:

建立同时测定土茯苓中(-)-表儿茶素、5-O-咖啡酰基莽草酸、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷和黄杞苷含量的高效液相色谱法。采用AgilentZorbaxSB-C18色谱柱(250mm×4.6mm,5μm),柱温35℃,流动相为乙腈(B)-0.05%磷酸(A)梯度洗脱,流速1.0mL·min-1,检测波长230nm,对土茯苓、短柱肖菝葜、华肖菝葜药材进行分析。测定的84份土茯苓药材中(-)-表儿茶素、5-O-咖啡酰基莽草酸、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷和黄杞苷的含量范围分别为trace~1.381,trace~9.913,trace~3.673,0.6706~27.08,trace~1.181,trace~4.833,trace~2.754mg·g-1。用建立的方法对土茯苓常见混淆品短柱肖菝葜、华肖菝葜进行分析,结果显示短柱肖菝葜、华肖菝葜药材中(-)-表儿茶素的含量范围分别为0.01163~0.06007mg·g-1和0.01583~0.08585mg·g-1;而其他6种成分均未检测到。该方法简便、准确,可作为土茯苓药材的质量控制方法。短柱肖菝葜和华肖菝葜的成分显著不同于土茯苓,不宜与土茯苓混用。

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