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穿心莲内酯对白念珠菌群感效应及相关毒力基因的影响

Keywords: 穿心莲内酯,白念珠菌,群感效应,法尼醇,酪醇

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Abstract:

目的:研究穿心莲内酯(andrographolide,AG)对白念珠菌群感效应(quorumsensing,QS)及相关毒力基因的影响.方法:气相色谱-质谱联用仪(GC-MS)检测AG干预白念珠菌不同时间后法尼醇和酪醇的含量变化;qRT-PCR检测QS调节的相关毒力基因CHK1,PBS2,HOG1的表达.结果:白念珠菌生长2h时法尼醇和酪醇分泌量较少,AG作用后变化不明显;12h后分泌量最高,24h回落.不同浓度AG干预后,法尼醇含量降低,酪醇升高,呈现剂量依赖性,其中1000mg·L-1AG效果均最明显.qRT-PCR结果显示,1000mg·L-1AG在2,12,24h时分别使CHK1下调2.375,3.330,4.043倍;PBS2下调2.010,4.210,4.760倍;HOG1变化不明显.结论:AG可抑制法尼醇的分泌及促进酪醇的分泌,下调QS相关的毒力基因CHK1,PBS2的表达.

References

[1]  Albuquerque P, Acasadevall A. Quorum sensing in fungi[J]. Med Mycol, 2012,50(4):337.
[2]  Miller M B, Bassler B L. Quorum sensing in bacteria[J]. Annu Rev Icrobiol,2001,55:165.
[3]  Ramage G, Walle K V, Wickes B L, et al. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms[J].Antimicrob Agents Chemother,2001,45(9):2475.
[4]  Selma T,Christina K,Andrea L,et al.In vitro activity of antifungal combinations against Candida albicans biofilms[J].J Antimicrob Chemother,2010,65(2):271.
[5]  Ramage G, Saville S P, Wickes B L, et al. Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule[J].Appl Environ Microbiol, 2002, 68(11): 5459.
[6]  Nickerson K W, Atkin A L, Hornby J M. Quorum sensing in dimorphic fungi Farnesol and beyond[J]. Appl Environ Microbiol,2006,72(6):3805.
[7]  Navarathna D H, Hornby J M, Krishnan N, et al. Effect of farnesol on a mouse model of systemic candidiasis, determined by use of a DPP3 knock-out mutant of Candida albicans[J]. Infec Immun, 2007,75(4):1609.
[8]  Chen H,Fujita M,Ferg Q, et a1.Tyrosol is a quorum-sensing Molecule in Candida albicans[J].Proc Natl Acad Sci USA,2004,101(14):5048.
[9]  陈晓君,谭军艳,马鸣,等.不同培养基对白念珠菌生物被膜产生法尼醇的影响[J].口腔生物医学,2012,3(2):68.
[10]  魏昕,吴观陵,刘卫红,等.白念珠菌的菌体密度与生物被膜形成及tyrosol分泌[J].中华微生物学和免疫学杂志,2010,30(4):344.
[11]  Cheetham J, Smith D A, da Silva Dantas A, et al. A single MAPKKK regulates the Hog1 MAPK pathway in the pathogenic fungus Candida albicans[J]. Mol Biol Cell,2007,18(11):4603.
[12]  陈美静.白念珠菌CHK突变株致病性研究[D].福州:福建医科大学,2009:17.
[13]  Chauhan, N,Calderone R. Two-component signal transduction proteins as potentialdrug targets in medically important fungi[J]. Infect Immun, 2008, 76(11): 4795.
[14]  Bahn Y S. Master and commander in fungal pathogens: the two-component system and the HOG signaling pathway[J]. Eukaryot Cell,2008, 7(12): 2017.
[15]  Deveau A, Piispane A E, Jackson A A, et al. Farnesol induces hydrogen peroxide resistance in Candida albicans yeast by inhibiting the Ras-cyclic AMP signaling pathway[J].Eukaryot Cell,2010,9(4):569.
[16]  Alonso-Monge R, Navarro-García F, Molero G, et al. Role of themitogen-activated protein kinaseHog1p inmorphogenesis and virulence of Candida albicans[J]. J Bacteriol,1999, 181(10):3058.
[17]  Cheetham J, MacCallum D M, Doris K S, et al. MAPKKK-independent regulation of the Hog1 stress-activated protei kinase in Candida albicans[J]. J Biol Chem, 2011,286(49):42002.
[18]  Popat R, Crusz S A, Diggle S P. The social behaviors of bacterial pathogens[J]. Br Med Bull, 2008, 87(1):63.
[19]  Hornby J M, Jensen E C, Lisec A D, et al. Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol[J]. Appl Environ Microbiol, 2001, 67(7): 2982.
[20]  严园园,施高翔,邵菁,等.穿心莲内酯及其衍生物抗感染研究近十年进展[J].中国中药杂志,2013,38(22):3819.

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