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ABA及其生物合成抑制剂对丹参毛状根酚酸类成分和关键酶的影响

Keywords: 丹参,毛状根,脱落酸,酚酸类物质

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Abstract:

目的:研究脱落酸(ABA)及其生物合成抑制剂氟啶酮(fluridone),对丹参毛状根酚酸类成分和关键酶的影响。方法:继代培养18d的丹参毛状根添加不同浓度的ABA及ABA与氟啶酮组合,处理1d后测定关键酶PAL和TAT活性;处理6d后测定不同处理的丹参毛状根中酚酸类物质的含量。结果:在一定浓度范围内,低浓度的ABA促进丹参毛状根的生长,高浓度的ABA抑制丹参毛状根的生长;ABA显著促进酚酸类物质的积累,对咖啡酸的诱导表现出正相关,但是迷迭香酸和丹酚酸B的效果,表现出在低浓度效果较好,随浓度增大,诱导效果降低,直到ABA浓度至200μmol·L-1,含量开始上升;当ABA与氟啶酮组合处理时,氟啶酮抑制ABA对丹参毛状根酚酸类的积累,但是有差异;不同浓度的ABA会不同程度的提高PAL和TAT酶的活性,添加ABA生物合成抑制剂,酶活性诱导效果降低。结论:ABA能诱导丹参毛状根中酚酸类化合物积累,同时也能激活合成相关酶的活性;ABA生物合成抑制剂氟啶酮不同程度抑制ABA的诱导效果。

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