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中国西南7种石斛植物活性内生真菌及其遗传关系

Keywords: 抑菌活性,琼脂扩散法,内生真菌,ITS序列分析,热带雨林植物,石斛

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Abstract:

目的:对中国西南地区7种石斛植物的内生真菌进行抗菌活性筛选,考察活性菌株之间的遗传关系。方法:采用组织块法分离真菌,琼脂扩散法进行抑菌活性筛选,通过rDNA的ITS序列为基础鉴定活性真菌,用邻接法(NJ)和最大简约法(MP)进行遗传关系考察。结果:从7种石斛属植物分离到98株真菌,它们对Escherichiacoli,Bacillussubtilis,Staphylococcusaureus,Candidaalbicans,Cryptococcusneoformans和Aspergillusfumigatus的抑制率分别是1.02%,102%,184%,1.02%,1.02%,10.2%,其中DL-3,DL-18,DL-21,DM-2,DG-10和DN-1显示出了对B.subtilis,S.aureus和A.fumigatus较强的生长抑制性。24株活性真菌隶属于7个目,11个属,14个种,NJ和MP系统树阐明活性菌之间的亲缘关系。结论:本研究对于考察珍惜濒危石斛属植物的内生真菌的生物活性以及它们之间的遗传关系具有重要的生态价值,为石斛根茎部活性真菌的研究提供参考。

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