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PCR增强剂对药材DNA分子鉴定的影响

Keywords: PCR增强剂,中药材,分子鉴定

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Abstract:

评价PCR增强剂对中药材DNA分子鉴定的影响,筛选获得适用于中药材DNA分子鉴定的PCR增强剂。文章分别利用CTAB法和碱裂解法提取180个中药材DNA样品,分析在PCR反应体系中使用PCR增强剂对通用引物ITS2,psbA-trnH,rbcL,matK,trnL-trnF片段PCR成功率的影响及实时荧光定量PCR反应体系中使用增强剂对PCR扩增效率的影响。结果表明BSA与PVP组合可以明显增加ITS2,psbA-trnH,rbcL片段的PCR成功率,且PCR增强剂也能消除位点特异性鉴别假阴性结果,提高分子鉴定的准确性,但降低了实时荧光定量PCR的扩增效率。本研究说明BSA和PVP作为PCR增强剂能增加中药材DNA的PCR扩增成功率和分子鉴别的准确性。

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