UPLC同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚、和厚朴酚的含量

目的:建立同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚以及和厚朴酚6个成分的UPLC分析方法。方法:采用ZorbaxEclipseC18柱,以乙腈(A)-0.05%磷酸溶液(B)为流动相,梯度洗脱(0~5min,17%~20%A,5~12min,20%~50%A,12~18min,50%~70%A,18~18.5min,70%~95%A,18.5~23.5min,95%A),流速0.42mL·min-1,检测波长220nm,柱温30℃。结果:甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、和厚朴酚以及厚朴酚进样量分别在0.0017~0.034,0.0034~0.068,0.0064~0.128,0.0128~0.256,0.0032~0.064,0.0064~0.128μg线性关系良好,平均加样回收率分别为103.3%,98.39%,98.29%,102.1%,98.45%,102.2%,RSD分别为2.1%,1.0%,1.5%,2.3%,0.90%,2.0%。结论:UPLC分离效果及重复性好,且快速简便,可作为藿香正气口服液的质量控制方法。