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粗壮润楠中的木脂素类成分

Keywords: 粗壮润楠,木脂素苷,木脂素苷元,抑制小鼠腹腔巨噬细胞分泌NO活性

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Abstract:

通过萃取、正相硅胶、SephadexLH-20、中压柱色谱以及反相HPLC柱色谱等多种分离方法相结合,从粗壮润楠树皮乙醇提物中分离得到18个木脂素类化合物;通过质谱和核磁共振等波谱学分析方法鉴定结构为:异落叶松脂素9'-O-β-D-吡喃木糖苷(1),5'-甲氧基异落叶松脂素9'-O-β-D-吡喃木糖苷(2),南烛木树脂酚9'-O-β-吡喃木糖苷(3),(+)-(8S,8'S)-4,4'-二羟基-3,3',5,5'-四甲氧基木脂烷-9,9'-二醇9-O-β-D-吡喃木糖苷(4),南烛木树脂酚(5),内消旋二氢愈创木脂酸(6),(+)-(8S,8'R)-3',4,4'-三羟基-3'-甲氧基木脂烷(7),(8S,8'R)-4'-羟基-3,3',4-三甲氧基木脂烷(8),(+)-愈创木素(9),异愈创木素(10),(-)-(7'R,8R,8'R)-4,4'-二羟基-3,3',5-三甲氧基-2,7'-环木脂烷(11),翼梗五味子木脂素B(12),(-)-(7S,7'S,8R,8'R)-4,4'-二羟基-3,3',5,5'-四甲氧基-7,7'-环氧木脂素-9,9'-二醇(13),(+)-(7R,8R,7'E)-4-羟基-3,5'-二甲氧基-7,4'-环氧-8,3'-氧新木脂素-7'-烯(14),甘密树皮素B(15),桢楠素I(16),(-)-松脂素(17)和(-)-丁香树脂酚(18)。以上化合物均为首次从该植物中分离得到。经体外活性筛选,在10μmol·L-1时,化合物17对小鼠腹腔巨噬细胞分泌NO具有较强的抑制活性,抑制率为72.2%。

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