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基于UPLC-TQ-MS考察不同树龄果用银杏叶萜内酯含量变化规律

Keywords: UPLC-TQ-MS,果用银杏叶,萜内酯,树龄

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Abstract:

目的:建立超高效液相色谱串联三重四级杆质谱法(UPLC-TQ-MS)同时测定银杏叶中4个萜内酯成分的含量。方法:采用ACQUITYTMUPLCBEHC18(2.1mm×100mm,1.7μm)色谱柱,以水(A)-甲醇(B)为流动相,等度洗脱(70%A),流速0.4mL·min-1,柱温30℃;WatersXevoTMTQ质谱仪以多反应离子监测(MRM)方式进行负离子检测。结果:各成分在5.0min内分离良好,在相应的浓度范围内与峰面积呈良好的线性关系,相关系数均>0.9903;加样回收率(n=6)为98.83%~103.9%,RSD均小于3%。不同树龄果用银杏叶中萜内酯的含量差异较大,低树龄银杏叶含总内酯较高,随着树龄的增长,内酯含量逐渐降低。结论:该方法简便、快速、重复性好、专属性强,适用于银杏叶中萜内酯类成分的快速定量分析。

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