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清火栀麦片中化学成分的SPE-HPLC-ESI-MSn快速识别与鉴定

Keywords: 高效液相色谱-电喷雾串联质谱,固相萃取,清火栀麦片,化学成分鉴定

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Abstract:

目的:建立一种固相萃取-高效液相色谱-电喷雾串联质谱(SPE-HPLC-ESI-MSn)分析方法,对清火栀麦片中主要的化学成分进行快速识别与鉴定。方法:根据HPLC-ESI-MSn负离子模式下的分子离子峰以及碎片离子获得化合物的结构信息,并通过与文献数据或对照品对照,确定化合物可能的结构。结果:通过对清火栀麦片及其组方药味进行比较分析,归属并鉴定了其中的39个化学成分,包括7个咖啡酰奎尼酸、7个环烯醚萜、6个二萜内酯、5个高异黄酮、13个甾体皂苷以及1个黄酮类成分。结论:该研究为鉴别清火栀麦片中的化学成分提供了一种简便、快速的方法。

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