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植物研究  2014 

蔷薇科6个属植物EST-SSR特征分析

DOI: 10.7525/j.issn.1673-5102.2014.06.016, PP. 810-815

Keywords: 蔷薇科,SSR,EST

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Abstract:

?EST-SSR是基于功能基因组开发的分子标记,由于其在基因组中广泛均匀分布,具有较好的种属通用性及与表型性状相关联等优点而得到广泛应用。本研究通过下载公共数据库中蔷薇科蔷薇属,苹果属,梨属,李属,草莓属和悬钩子属的EST序列,运用生物信息学的方法,调查6个属中SSR的分布规律及特点。结果表明6个属间SSR分布频率差别较大,从悬钩子属的10.94%到梨属的43.49%。二碱基和三碱基重复类型在6个属中占据优势,其中二碱基重复类型主要为AG/CT、GA/TC及AT/TA,而三碱基重复类型在6个属中差异较大,优势类型为AGA/TCT、GAA/TTC和AAG/CTT及CAC/GTG和ACA/TGT等,显示出较好的属间特异性。该结果对于研究蔷薇科植物的分子系统发育与进化,种质资源鉴定,遗传多样性以及遗传作图提供了研究平台。

References

[1]  1.Powell W,Machray G C,Provan J.Polymorphism revealed by simple sequence repeats[J].Trends Plant Science,1996,1:215-222.
[2]  2.王彩虹,田义轲,赵静.来自苹果的SSRs在蔷薇科植物资源上的通用性分析[J].园艺学报,2005,32(3):500-502.
[3]  3.Varshney R K,Graner A,Sorrells M E.Genic microsatellite markers in plants:features and applications[J].Trends in Biotechnology,2005,23(1):48-55.
[4]  4.Li S X,Yin T M.Map and analysis of microsatellites in genome of Populus:the first sequenced perennial plant[J].Science in China C:Life Sciences,2006,50(5):690-699.
[5]  5.李淑娴,张新叶,王英亚,等.桉树EST序列中微卫星含量及相关特征[J].植物学报,2010,45(3):363-371.
[6]  6.Talia P,Nishinakamasu V,Hopp H E,et al.Genetic mapping of EST-SSR,SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map[J].Electronic Journal of Biotechnology,2010,13(6):7-8.
[7]  7.Celton J M,Tustin D S,Chagne D,et al.Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences[J].Tree Genetics & Genomes,2009,5(1):93-107.
[8]  8.Riju A,Rajesh M K,Sherin P T P F,et al.Mining of expressed sequence tag libraries of cacao for microsatellite markers using five computational tools[J].Journal of genetics,2009,88(2):217-225.
[9]  9.Li X,Shangguan L,Song C,et al.Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers[J].BMC genetics,2010,11(1):66.
[10]  10.俞德浚.中国植物志:第36卷[M].北京:科学出版社,1974.
[11]  11.俞德浚.蔷薇科植物的起源和进化[J].植物分类学报,1984,22(6):431-444.
[12]  12.Watson L,Dallwitz M J.The families of angiosperms:automated descriptions,with interactive identification and information retrieval[J].Australian Systematic Botany,1991,4(4):681-695.
[13]  13.Huang X,Madan A.CAP3:A DNA Sequence Assembly Program[J].Genome Research,1999,9:868-877.
[14]  14.Thiel T.MISA-Microsatellite Identification Tool,Version 1.0.2001,http://pgrc.ipk-gatersleben.de/misa/misa.html
[15]  15.黄启星,左娇,孔华,等.11种热带植物EST-SSR标记的开发和多样性分析[J].热带作物学报,2012,33(7):1208-1214.
[16]  16.刘峰,王运生,田雪亮,等.辣椒转录组SSR挖掘及其多态性分析[J].园艺学报,2012,39(1):168-174.
[17]  17.段紫英,胡丽芳,黄长干,等.黄瓜EST中SSR的分布特征分析[J].广东农业科学,2012,39(22):160-161.
[18]  18.Yan M M,Dai X G,Li S X,et al.A Meta-Analysis of EST-SSR Sequences in the Genomes of Pine,Poplar and Eucalyptus[J].Genomics and Applied Biology,2011,30(1):1-7.
[19]  19.安泽伟,赵彦宏,程汉,等.橡胶树EST-SSR标记的开发与应用[J].遗传,2009,31(3):311-319.
[20]  20.Liang X,Chen X,Hong Y,et al.Utility of EST-derived SSR in cultivated peanut( Arachis hypogaea L.)and Arachis wild species[J].BMC Plant Biology,2009,9:35-48.
[21]  21.Gupta P K,Balyan H S,Sharma P C,et al.Microsatellites in plants:A new class of molecular marker[J].Current sciences,1996,70(1):45-54.
[22]  22.Jung S,Abbott A,Jesudurai C,et al.Frequency,type,distribution and annotation of simple sequence repeats in Rosaceae ESTs[J].Functional & integrative genomics,2005,5(3):136-143.
[23]  23.Gao L F,Tang J,Li H,et al.Analysis of microsatellites in major crops assessed by computational and experimental approaches[J].Molecular Breed,2003,113:163-185.
[24]  24.Nicot N,Chiquet V,Gandon B,et al.Study of simple sequence repeat(SSR) markers from wheat expressed sequence tags(ESTs)[J].Theory of Apply Genets,2004,109:800-805.
[25]  25.Aggarwal R K,Hendre P S,Varshney R K,et al.Identification,characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species[J].Theoretical and Applied Genetics,2007,114:359-372.
[26]  26.Schorderet D F,Gartler S M.Analysis of CpG suppression in methylated and nonmethylated species[J].PNAS,1992,89:957-961.
[27]  27.Akkaya M S,Bhagwat A A,Cregan P B.Length polymorphisms of simple sequence repeat DNA in soybean[J].Genetics,1992,132:1131-1139.
[28]  28.Kumpatla S P,Mukhopadhyay S.Mining and survey of simple sequence repeats in expressed sequence tags of dicotyledonous species[J].Genome,2005,48:985-998.
[29]  29.Zhang D X.Lepidopteran microsatellite DNA:redundant but promising[J].Trends in Ecology and Evolution,2004,19:507-509.
[30]  30.Yadav H K,Ranjan A,Asif M H,et al.EST-derived SSR markers in Jatropha curcas L.:development,characterization,polymorphism,and transferability across the species/genera[J].Tree Genetics & Genomes,2011,7(1):207-219.
[31]  31.Clement C R.1492 and the loss of Amazonian crop genetic resources.Ⅰ.The relation between domestication and human population decline[J].Economic.Botany,1999,53:188-202.
[32]  32.Pickersgill B.Domestication of plants in the Americas:insights from Mendelian and molecular genetics[J].Annual Botany,2007,100:925-940.

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