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植物研究  2015 

基于叶绿体DNAndhA和nrDNAITS序列变异分析栽培柿及其近缘种亲缘关系

DOI: 10.7525/j.issn.1673-5102.2015.04.007, PP. 515-520

Keywords: cpDNA,ndhA,ITS,,亲缘关系

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Abstract:

?以柿属植物(Diospyrosspp.)中与柿近缘的8种共30个基因型为试材,进行核糖体DNA(nrDNA)内转录间隔区(ITS)和叶绿体DNAndhA序列变异分析,并通过软件计算两个序列及合并后的进化模型,依据进化模型采用ML法(maximumlikelihoodmethod)分析进化关系。为进一步弄清柿属植物种间亲缘关系和供试柿(DiospyroskakiThunb.)种内分子差异提供了理论依据。结果表明:(1)ndhA序列长度变异范围在1492~1511,14个信息位点;ITS序列长度变异范围在660~761,56个信息位点。ITS、ndhA和ndhA+ITS(ndhA和ITS合并)最适碱基进化模型分别为(TrN+I+G)、(F81+I)和(GTR+I+G)。综合ITS和ndhA序列分析表明:柿与油柿和云南野毛柿亲缘关系最近,与美洲柿和乌柿最远。(2)21份柿品种材料的ITS长度均为730,包括4个变异位点,据此4个变异位点对供试柿种内21个品种进行聚类分析。研究认为,ndhA和ITS能较清楚解释了柿与其近缘种间的亲缘关系,并通过柿品种ITS的差异位点分析鉴别出栽培柿种内的差异。

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