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植物研究  2015 

小黑杨FLC基因的克隆及功能解析

DOI: 10.7525/j.issn.1673-5102.2015.03.007, PP. 363-369

Keywords: 小黑杨,FLC基因,开花调控,春化

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Abstract:

?MADS-box家族蛋白是一类重要的调控开花的转录因子,FLC是其家族成员的编码基因之一。FLC在调节开花过程,其功能的发挥受多个途径的调控。本研究利用RT-PCR方法在小黑杨雄花芽中克隆出FLC基因的全长cDNA序列PnFLC。该基因的开放读码框为726bp,编码241个氨基酸残基组成的分子量为27.559kD的蛋白质,蛋白质的等电点为9.37。实时定量荧光PCR结果显示,春化能够使PnFLC在小黑杨根、茎、叶各组织中表达量下调57.9%~84%;启动子GUS染色结果表明,PnFLC启动子在分裂活跃的组织中活性较高。拟南芥的遗传转化结果显示,PnFLC可以调控AtAP1、AtSOC1和AtFT基因的表达,同时延迟拟南芥的开花时间。

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