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植物研究  2015 

柽柳中一种新型硫氧还蛋白H基因的克隆与鉴定

DOI: 10.7525/j.issn.1673-5102.2015.03.004, PP. 340-346

Keywords: 硫氧还蛋白,基因表达,非生物胁迫应答,刚毛柽柳

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Abstract:

?硫氧还蛋白对维持生物体内稳定的氧化还原状态发挥着重要作用。为了研究硫氧还蛋白在非生物胁迫条件下的功能,我们从柽柳的cDNA文库中分离出来一条新的硫氧还蛋白cDNA序列,命名为ThTrx1。生物信息学分析表明,ThTrx1是硫氧还蛋白h亚家族的一个新成员,属于h1亚族。我们采用实时定量RT-PCR的方法检测在不同非生物胁迫(NaCl,PEG,CdCl2,低温和ABA)下,ThTrx1基因在刚毛柽柳根和叶中的表达。结果表明,在NaCl,PEG,CdCl2和ABA处理条件下,ThTrx1在柽柳根和叶中的mRNA水平都是上调表达。而在低温胁迫下,ThTrx1下调表达。我们通过Thrx1基因在大肠杆菌中的表达来分析它对于细菌生长和耐受性的影响。结果表明,含重组质粒的大肠杆菌(pET32a-Trx1)与对照相比,对于盐,金属离子和干旱胁迫体现出更高的耐受性。我们的结果表明,该ThTrx1是参与非生物胁迫应答,并且受依赖ABA信号转导途径的调节。

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