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植物学报  2015 

非洲菊微管相关蛋白基因GMAP65-1功能分析

DOI: 10.3724/SP.J.1259.2015.00012, PP. 12-21

Keywords: cDNA克隆,非洲菊,微管相关蛋白,形态建成

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Abstract:

?微管相关蛋白在植物生长发育过程中发挥重要作用。利用反转录聚合酶链式反应(RT-PCR)和快速扩增cDNA末端(RACE)技术对非洲菊(Gerberahybrida)体内微管相关蛋白GMAP65-1基因进行了克隆,获得的基因全长1883bp,包含1740bp的完整开放阅读框(ORF)。表达模式研究表明,该基因在非洲菊幼嫩的根、叶及花中均有较高的表达,且受到赤霉素(GA)诱导显著上调。构建GMAP65-1超表达载体,经异源转化拟南芥(Arabidopsisthaliana)后筛选获得纯合株系,对纯合株系进行表型观察。结果表明,GMAP65-1超表达植株的叶片及花瓣面积增大,暗示该基因参与叶片及花瓣的形态建成。研究结果为花卉分子育种提供了理论依据及基因资源。

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