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植物学报  2013 

仙鹤藓的孢子萌发及愈伤组织诱导

DOI: 10.3724/SP.J.1259.2013.00651, PP. 651-657

Keywords: 仙鹤藓,愈伤组织,孢子萌发

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Abstract:

?通过对采自河北雾灵山海拔1500m的仙鹤藓(Atrichumundulatum)的孢子萌发以及原丝体发育的观察,发现仙鹤藓孢子无休眠现象,孢子接种3天左右萌发;其原丝体发育分为绿丝体和轴丝体两个阶段。扩大培养实验结果表明,仙鹤藓茎叶体在添加2%葡萄糖的MS培养基上,置于25°C/20°C、14小时光照/10小时黑暗、36μmol·m–2·s–1条件下培养,产生新生茎叶体最多,且茎叶体长势最好,可以获得大量无菌材料。仙鹤藓愈伤组织诱导实验显示,形成愈伤组织的最佳培养基为添加2%葡萄糖和1.0mg·L–16-BA的MS培养基。

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