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植物学报  2014 

木薯SYP基因的序列特征及表达分析

DOI: 10.3724/SP.J.1259.2014.00063, PP. 63-77

Keywords: 木薯,SYP基因,进化树,基因表达

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Abstract:

?植物突触融合蛋白(SYP)是一类与植物细胞内囊泡介导转运有关的蛋白。部分SYP基因与植物对生物和非生物胁迫的响应有关。该文利用生物信息学工具分析了木薯(Manihotesculenta)SYP基因及其蛋白结构、核苷酸多态性和系统进化关系,并利用RT-PCR技术检测了木薯不同组织中SYP基因的表达。结果表明,木薯SYP基因及其蛋白结构均具有明显的规律性和家族成员间的保守性;SYP基因的cDNA在基因间以及不同品种间具高度一致性,核苷酸变异以同义替换为主。进化分析表明,植物SYP基因可分为2个亚家族,木薯SYP基因倾向于与蓖麻(Ricinuscommunis)SYP基因聚在进化树同一分支的末端。半定量RT-PCR分析表明,5个木薯SYP家族成员具有组织特异性。上述研究结果为木薯SYP基因功能研究和功能单核苷酸标记的开发奠定了重要基础。

References

[1]  Bassham DC, Sanderfoot AA, Kovaleva V, Zheng H, Raikhel NV (2000). AtVPS45 complex formation at the trans-Golgi network. Mol Biol Cell 11, 2251-2265.
[2]  Chen Y, Shin YK, Bassham DC (2005). YKT6 is a core constituent of membrane fusion machineries at the Arabidopsis trans-Golgi network. J Mol Biol 350, 92-101
[3]  Collins NC, Hans T, Lipka V, Bau S, Kombrink E, Qiu J, Hückelhoven R, Stein M, Freialdenhoven A, Somerville SC, Paul S (2003). SNARE-protein-mediated disease resistance at the plant cell wall. Nature 425, 973-977
[4]  Enami K, IChikawa M, Uemura T, Kutsuna N, Hasezawa S, Nakagawa T, Nakano A, Sato MH (2009). Differential expression control and polarized distribution of plasmamembrane-resident SYP1 SNAREs. Plant Cell Physiol 50,280-289.
[5]  Hebsgaard SM, Korning PG, Tolstrup N, Engelbrecht J, Rouze P, Brunak S (1996). Splice site prediction in Arabidopsis thaliana DNA by combining local and global sequence information. Nucl Acids Res 24, 3439-3452.
[6]  Heese M, Gansel X, Sticher L, Wick P, Grebe M, Granier F, Jürgens G (2001). Functional characterization of the KNOLLE-interacting t-SNARE AtSNAP33 and its role in plant cytokinesis. J Cell Biol 155, 239-249.
[7]  Honsbei NA, Sokolovski S, Grefen C, Campa NP, Pratelli R, Paneque M, Chen Z, Johansson I, Blatt MR (2009). A tripartite SNARE-K+ channel complex mediates in channel dependent K + nutrition in Arabidops is. Plant Cell 21, 2859-2877.
[8]  Jones DT (1999). Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol 292, 195-202.
[9]  Jones DT (2007). Improving the accuracy of transmembrane protein topology prediction using evolutionary information. Bioinformatics 23: 538-544
[10]  Jones DT, Taylor WR, Thornton JM (1994). A Model Recognition Approach to the Prediction of All-Helical Membrane Protein Structure and Topology. Biochem 33, 3038-3049.
[11]  Kalde M, Nühse TS, Findlay K, Peck SC (2007). The syntaxin SYP132 contributes to plant resistance against bacteria and secretion of pathogenesis-related protein 1. Proc Natl Acad Sci USA 104, 11850-11855.
[12]  Arnold K, Bordoli L, Kopp J, Schwede T (2006). The SWISS-MODEL Workspace: A web-based environment for protein structure homology modelling. Bioinformatics, 22,195-201.
[13]  Assaad FF, Qiu JL, Youngs H, Ehrhardt D, ZimmerliL,Kalde M, Wanner G, Peck SC, Edwards H, RamonellK,Somerville CR, Thordal-Christensen H (2004). The PEN1 syntaxin defines a novel cellular compartment uponfungal attack and is required for the timely assembly of papillae. Mol Biol Cell 15, s5118-5129.
[14]  Bailey TL, Bodén M, Buske FA, Frith M, Grant CE, Clementi L, Ren JY, Li WW, Noble WS (2009). MEME SUITE: tools for motif discovery and searching. Nucl Acids Res 37, 202-208.
[15]  Bailey TL, Elkan C (1994). Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36, AAAI Press, Menlo Park, California, 1994.
[16]  Brunak S, Engelbrecht J, Knudsen S (1991). Prediction of Human mRNA donor and scceptor sites from the DNA Sequence, J Mol Bio 220, 49-65
[17]  Kwon C, Neu C, Pajonk S, Yun HS, Lipka U, Humphry M, Bau S, Straus M, Kwaaitaal M, Rampelt H, Kasmi FE, Jurgens G, Parker J, Panstruga R, Lipka V, Schulze-Lefert P (2008). Co-option of a default secretory pathway for plant immune responses. Nature 451, 835-840.
[18]  Leyman B, Geelen D, Quintero FJ, Blatt MR (1999). A tobacco syntaxin with a role in hormonal control of guard cell ion channels. Science 283, 537-540.
[19]  Librado P and Rozas J (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25,1451-1452. doi: 10.1093/bioinformatics/btp187.
[20]  Nugent T, Jones DT (2009). Transmembrane protein topology prediction using support vector machines. BMC Bioinformatics. 10, 159. Epub
[21]  Nühse TS, Boller T, Peck SC (2003). A plasma membrane syntaxin is phosphorylated in response to the bacterial elicitor flagellin. The Journal of BiologicalChemistry, 278, 45248-45254.
[22]  Patelli R, Sutter JU, Blatt MR (2004). A new catch in the SNARE. Trends Plant Sci, 9(4), 187-195.
[23]  Rancour DM, Dickey CE, Park S, Bednarek SY (2002). Characterization of AtCDC48 evidence for multiple membrane fusion mechanisms at the plane ofcell division in plants. Plant Physiol 130, 1241-1253.
[24]  Ruan MB, Zhao YT, Meng ZH, WangXJ, Yang WC(2009). Conserved miRNA analysis in Gossypiumhirsutum through small RNA sequencing. Genomics, 94(4), 263-268.
[25]  Sanderfoot AA, Assaad FF, Raikhel NV (2000). The Arabidopsis genome an abundance of soluble Nethylmaleimide-sensitive factor adaptor protein receptors. Plant Physiol 124, 1558-1569.
[26]  Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV (2001b). Interactions between syntaxins identify at leastfive SNARE complexes within the Golgi/prevacuolar system stimulation. Plant Physiol 132, 343-351
[27]  Sanderfoot AA, Pilgrim M, Adam L, Raikhel NV (2001a). Disruption of individual members of Arabidopsis syntaxin gene families indicates each has essential functions. Plant Cell 13, 659-666.
[28]  Sokolovski S, Hills A, Gay RA, Blatt MR (2008). Functional interaction of the SNARE protein NtSyp121 in Ca2+ channel gating, Ca2+ transients and ABA signalling of stomatal guard cells. Mol. Plant 1, 347-358.
[29]  Sutter JU, Campanoni P, Tyrrell M, Blatt MR (2006) Selective mobility and sensitivity to SNAREs is exhibited by the ArabidopsisKAT1 K+ channel at the plasma membrane. Plant Cell 18, 935-954
[30]  Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25, 4876-4882.
[31]  Wick P, Gansel X, Oulevey C, Page V, Studer I, Dürst M, Sticher L (2003). The expression of the t-SNARE AtSNAP33 is induced by pathogens and mechanical of the Arabidopsis cell. Mol Biol Cell 12, 3733-3743.
[32]  Zheng H, Bednarek SY, Sanderfoot AA, Alonso J, Ecker JR, Raikhel NV (2002). NPSN11 is a cell plate associated SNARE protein that interacts with the syntaxin KNOLLE. Plant Physiol 129, 530-539
[33]  Zhu J, Gong Z, Zhang C, Song C, Damsz B, Inan G, Koiwa H, Zhu J, Hasegawa PM, Bressan RA (2002). OSM1/SYP61: a syntaxin protein in Arabidopsis controls abscisic acid-mediated and non-abscisic acid-mediated responses to abiotic stress. Plant Cell, 14, 3009-3028.

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