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植物学报  2011 

核基质结合区在番茄转基因表达调控中的应用

DOI: 10.3724/SP.J.1259.2011.00688, PP. 688-693

Keywords: 农杆菌转化,核基质结合区,番茄

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Abstract:

?核基质结合区(matrixattachmentregion,MAR)的应用是提高植物基因转化和表达效率的有效方法之一。将烟草(Nicotianatabacum)核基质结合区TM2构建在植物表达载体pBI121上报告基因GUSA表达盒和选择标记基因NPTII表达盒的两侧翼,利用农杆菌介导的子叶浸染转化番茄(Lycopersiconesculentum)。结果表明,MAR序列能够显著提高转基因植株的转化效率和转基因的表达水平。不同长度的CaMV35S启动子比较表明,TM2的调控活性依赖于启动子的存在,并且具有一定的功能重叠。热诱导型启动子的研究表明,TM2仅提高热诱导的表达强度,而不改变启动子的热诱导表达调控特性。TM2的表达调控特性符合转基因的表达要求,该MAR序列可广泛应用于各种植物的基因工程中。

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