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植物学报  2011 

一个水稻矮秆突变体的遗传分析及基因定位

DOI: 10.3724/SP.J.1259.2011.00617, PP. 617-624

Keywords: 矮秆突变体,基因定位,遗传分析,水稻

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Abstract:

?水稻(Oryzasativa)是我国重要的粮食作物之一。水稻矮秆材料的引入掀起了第1次“绿色革命”。但近年来,在水稻育种中矮生基因遗传单一的问题越来越突出,已经严重影响到水稻产量的持续提高。利用60Co-γ射线辐照籼稻亲本材料M804获得了一个性状能够稳定遗传的矮秆突变体MU101。对该矮秆突变体和台粳16号杂交获得的F2代的遗传分析表明,该矮秆性状受1对隐性单基因控制,并暂命名为ds1。利用已有的SSR分子标记将DS1基因定位在水稻第5号染色体上,通过扩大群体和开发新的Indel标记,进一步将DS1基因定位在2个Indel标记之间,两者间的物理距离大约为384kb。该研究为DS1基因的克隆及其在生产中的应用奠定了基础。

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