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植物学报  2011 

水稻短根毛突变体Ossrh2的表型分析与基因定位

DOI: 10.3724/SP.J.1259.2011.00625, PP. 625-631

Keywords: 基因定位,遗传分析,水稻,短根毛突变体

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Abstract:

?在粳稻品种中花11为遗传背景的T-DNA突变体库中筛选获得一个遗传稳定的水稻(Oryzasativa)短根毛突变体Ossrh2(Oryzasativashortroothair2)。突变体在苗期表现为根毛数量减少,为野生型的61.4%,根毛长度明显变短,只有野生型的22.8%,同时根毛增粗,根毛形态也发生了变异,局部扭曲膨胀和分叉,除此之外突变体的地上部和根部生长情况与野生型相比没有显著差异。遗传分析表明,该突变性状受1对隐性单基因控制。通过对突变体T2和F2代的分子检测发现,该突变体表型非T-DNA插入引起。利用Ossrh2纯合体和籼稻品种Kasalath杂交构建的F2群体对OsSRH2进行基因定位,发现其与第10号染色体短臂上的SSR(simplesequencerepeat)标记RM6370和RM474连锁,遗传距离分别为1.1cM和3.0cM。通过在两标记间发展3个新的STS(sequence-taggedsite)标记,将OsSRH2基因定位于标记S1227和S1531之间,物理距离约为304kb,为进一步克隆OsSRH2打下了基础。

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